8ZDA

Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in a catalytically active state

Summary for 8ZDA

• Entry DOI: 10.2210/pdb8zda/pdb
• EMDB information: 39954
• Descriptor: CRISPR-associated endonuclease Cas9, sgRNA, Target DNA strand1, ... (7 entities in total)
• Functional Keywords: crispr-cas9, genome engineering, hydrolase-rna-dna complex, dna binding protein
• Biological source: Staphylococcus aureus; More
• Total number of polymer chains: 5
• Total formula weight: 182927.72

Authors

Omura, S.N.,Nakagawa, R.,Yamashita, K.,Nishimasu, H.,Nureki, O. (deposition date: 2024-05-01, release date: 2025-11-05)

• Primary citation: Omura, S.N.,Nakagawa, R.,Kajimoto, S.,Okazaki, S.,Ishiguro, S.,Mori, H.,Kashiwakura, Y.,Hiramoto, T.,Hirano, H.,Yamashita, K.,Jividen, K.,Tsai, S.Q.,Yachie, N.,Ohmori, T.,Nishimasu, H.,Nureki, O.; Molecular engineering and structural mechanism of the compact CRISPR-Cas9 nuclease; To Be Published,

Experimental method

ELECTRON MICROSCOPY (3.14 Å)