8ZDA
Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in a catalytically active state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1056 | 124305.9 | 1 | UniProt (J7RUA5) | Staphylococcus aureus | SaCas9 |
| 2 | B (B) | sgRNA | polymer | 99 | 31892.9 | 1 | Staphylococcus aureus | ||
| 3 | C (C) | Target DNA strand1 | polymer | 17 | 5267.6 | 1 | Staphylococcus aureus | ||
| 4 | D (D) | Non-target DNA strand | polymer | 43 | 13386.0 | 1 | Staphylococcus aureus | ||
| 5 | E (E) | Target DNA strand2 | polymer | 26 | 8002.5 | 1 | Staphylococcus aureus | ||
| 6 | F, G, H (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) PubChem (888) | |||
| 7 | I (A) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 1053 (UniProt: J7RUA5)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 413 | Asn 413 | conflict |
| Ala 420 | Arg 420 | conflict |
| Lys 782 | Glu 782 | conflict |
| Arg 800 | Leu 800 | conflict |
| Lys 927 | Thr 927 | conflict |
| Asn 929 | Lys 929 | conflict |
| Arg 968 | Asn 968 | conflict |
| Ala 985 | Asn 985 | conflict |
| Ser 986 | Asn 986 | conflict |
| Ala 991 | Arg 991 | conflict |
| Phe 1017 | Ile 1017 | conflict |
| Ser 1021 | Ala 1021 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 182854.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 72.9 | |
| All* | Total formula weight | 182927.7 |
