8ZDA
Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in a catalytically active state
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.14 |
| Software | Servalcat |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
