8YP6

Cryo-EM map of 30S ribosomal subunit in complex with MetAP1c of Mycobacterium smegmatis

Summary for 8YP6

• Entry DOI: 10.2210/pdb8yp6/pdb
• EMDB information: 39462
• Descriptor: 16S rRNA, Small ribosomal subunit protein uS11, Small ribosomal subunit protein uS12, ... (20 entities in total)
• Functional Keywords: methionine aminopeptidase type1c, anti-association factor, inter-subunit face, ribosome
• Biological source: Mycolicibacterium smegmatis MC2 155; More
• Total number of polymer chains: 20
• Total formula weight: 763136.62

Authors

Banerjee, A.,Srinivasan, K.,Sengupta, J. (deposition date: 2024-03-15, release date: 2025-03-19, Last modification date: 2025-10-01)

Primary citation

Banerjee, A.,Srinivasan, K.,Sengupta, J.
Mycobacterial Methionine Aminopeptidase Type 1c Moonlights as an Anti-association Factor on the 30S Ribosomal Subunit.
J.Mol.Biol., 437:169230-169230, 2025

PubMed Abstract: Methionine aminopeptidase (MetAP) is a vital metalloprotease that plays a crucial role in protein synthesis by binding to the 70S ribosome at the peptide exit tunnel and removing the N-terminal methionine from nascent polypeptide chains. In Escherichia coli, a single subclass of type 1 MetAP is present, whereas mycobacteria possess two subclasses, MetAP1a and MetAP1c. The key difference between these two is the presence of an additional 40 amino acid-long N-terminal extension in MetAP1c, which may contribute to distinct functional properties. In this study, we have uncovered a previously unrecognized "moonlighting" function of MetAP1c in mycobacteria. Interestingly, our results show that MetAP1c expression is specifically enhanced during the stationary phase of bacterial growth. Moreover, we identify a unique interaction between MetAP1c and the 30S ribosomal subunit, revealing its distinctive affinity for the small subunit. A 4.7 Å cryo-EM map of the Mycobacterium smegmatis MetAP1c-30S subunit complex demonstrates for the first time that MetAP1c binds at the inter-subunit face of the 30S subunit head region. The binding of MetAP1c induces conformational changes in the 30S subunit, impairing its ability to associate with the 50S subunit, thus imparting an anti-association property to MetAP1c. To further understand the role of the N-terminal extension, we constructed two mutant variants of MetAP1c, which confirmed its critical involvement in this moonlighting function. This anti-association activity of MetAP1c is likely one of the energy conservation mechanisms in mycobacteria where MetAP1c is involved in translation down regulation during stationary phase.

PubMed: 40441414
DOI: 10.1016/j.jmb.2025.169230

Experimental method

ELECTRON MICROSCOPY (4.7 Å)