8TXY

X-ray crystal structure of JRD-SIK1/2i-3 bound to a MARK2-SIK2 chimera

Summary for 8TXY

• Entry DOI: 10.2210/pdb8txy/pdb
• Descriptor: Serine/threonine-protein kinase MARK2, DI(HYDROXYETHYL)ETHER, N-[(5P,8R)-5-(2-cyano-5-{[(3R)-1-methylpyrrolidin-3-yl]methoxy}pyridin-4-yl)pyrazolo[1,5-a]pyridin-2-yl]cyclopropanecarboxamide, ... (5 entities in total)
• Functional Keywords: kinase, enzyme, chimera, inhibitor, biosynthetic protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 74692.41

Authors

Raymond, D.D.,Lemke, C.T.,Shaffer, P.L.,Collins, B.,Steele, R.,Seierstad, M. (deposition date: 2023-08-24, release date: 2024-01-10, Last modification date: 2024-11-20)

Primary citation

Babbe, H.,Sundberg, T.B.,Tichenor, M.,Seierstad, M.,Bacani, G.,Berstler, J.,Chai, W.,Chang, L.,Chung, M.,Coe, K.,Collins, B.,Finley, M.,Guletsky, A.,Lemke, C.T.,Mak, P.A.,Mathur, A.,Mercado-Marin, E.V.,Metkar, S.,Raymond, D.D.,Rives, M.L.,Rizzolio, M.,Shaffer, P.L.,Smith, R.,Smith, J.,Steele, R.,Steffens, H.,Suarez, J.,Tian, G.,Majewski, N.,Volak, L.P.,Wei, J.,Desai, P.T.,Ong, L.L.,Koudriakova, T.,Goldberg, S.D.,Hirst, G.,Kaushik, V.K.,Ort, T.,Seth, N.,Graham, D.B.,Plevy, S.,Venable, J.D.,Xavier, R.J.,Towne, J.E.
Identification of highly selective SIK1/2 inhibitors that modulate innate immune activation and suppress intestinal inflammation.
Proc.Natl.Acad.Sci.USA, 121:e2307086120-e2307086120, 2024

PubMed Abstract: The salt-inducible kinases (SIK) 1-3 are key regulators of pro- versus anti-inflammatory cytokine responses during innate immune activation. The lack of highly SIK-family or SIK isoform-selective inhibitors suitable for repeat, oral dosing has limited the study of the optimal SIK isoform selectivity profile for suppressing inflammation in vivo. To overcome this challenge, we devised a structure-based design strategy for developing potent SIK inhibitors that are highly selective against other kinases by engaging two differentiating features of the SIK catalytic site. This effort resulted in SIK1/2-selective probes that inhibit key intracellular proximal signaling events including reducing phosphorylation of the SIK substrate cAMP response element binding protein (CREB) regulated transcription coactivator 3 (CRTC3) as detected with an internally generated phospho-Ser329-CRTC3-specific antibody. These inhibitors also suppress production of pro-inflammatory cytokines while inducing anti-inflammatory interleukin-10 in activated human and murine myeloid cells and in mice following a lipopolysaccharide challenge. Oral dosing of these compounds ameliorates disease in a murine colitis model. These findings define an approach to generate highly selective SIK1/2 inhibitors and establish that targeting these isoforms may be a useful strategy to suppress pathological inflammation.

PubMed: 38147543
DOI: 10.1073/pnas.2307086120

Experimental method

X-RAY DIFFRACTION (2.1 Å)