8TB0

Cryo-EM Structure of GPR61-G protein complex stabilized by scFv16

Summary for 8TB0

• Entry DOI: 10.2210/pdb8tb0/pdb
• EMDB information: 41144
• Descriptor: Single-chain Fv16, GPR61 fused to dominant negative G alpha S/I N18 chimera, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, ... (4 entities in total)
• Functional Keywords: gpr61, gpcr, signaling complex, signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 185266.89

Authors

Lees, J.A.,Dias, J.M.,Han, S. (deposition date: 2023-06-28, release date: 2023-10-04)

• Primary citation: Lees, J.A.,Dias, J.M.,Rajamohan, F.,Fortin, J.P.,O'Connor, R.,Kong, J.X.,Hughes, E.A.G.,Fisher, E.L.,Tuttle, J.B.,Lovett, G.,Kormos, B.L.,Unwalla, R.J.,Zhang, L.,Dechert Schmitt, A.M.,Zhou, D.,Moran, M.,Stevens, K.A.,Fennell, K.F.,Varghese, A.E.,Maxwell, A.,Cote, E.E.,Zhang, Y.,Han, S.; An inverse agonist of orphan receptor GPR61 acts by a G protein-competitive allosteric mechanism.; Nat Commun, 14:5938-5938, 2023; PubMed: 37741852; DOI: 10.1038/s41467-023-41646-3

Experimental method

ELECTRON MICROSCOPY (3.47 Å)