8TB0
Cryo-EM Structure of GPR61-G protein complex stabilized by scFv16
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | F | Single-chain Fv16 | polymer | 269 | 28813.0 | 1 | Homo sapiens (human) | ||
| 2 | R | GPR61 fused to dominant negative G alpha S/I N18 chimera | polymer | 995 | 111175.8 | 1 | UniProt (Q9BZJ8) UniProt (P63092) Pfam (PF00001) Pfam (PF00503) Pfam (PF07361) UniProt (by SIFTS) (P0ABE7) In PDB | Escherichia coli | Biogenic amine receptor-like G-protein coupled receptor,Adenylate cyclase-stimulating G alpha protein |
| 3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
Sequence modifications
R: -140 - 1 (PDB: 8TB0)
R: 2 - 1446 (UniProt: Q9BZJ8)
R: 1481 - 1849 (UniProt: P63092)
R: 2 - 1446 (UniProt: Q9BZJ8)
| PDB | External Database | Details |
|---|---|---|
| Gly 1447 | - | linker |
| Ser 1448 | - | linker |
| Ser 1449 | - | linker |
| Gly 1450 | - | linker |
| Ser 1451 | - | linker |
| Ser 1452 | - | linker |
| Gly 1453 | - | linker |
| Ser 1454 | - | linker |
| Ser 1455 | - | linker |
| Glu 1456 | - | linker |
| Asn 1457 | - | linker |
| Leu 1458 | - | linker |
| Tyr 1459 | - | linker |
| Phe 1460 | - | linker |
| Gln 1461 | - | linker |
| Gly 1462 | - | linker |
| Ser 1463 | - | linker |
| Gly 1464 | - | linker |
| Cys 1465 | - | linker |
| Thr 1466 | - | linker |
| Leu 1467 | - | linker |
| Ser 1468 | - | linker |
| Ala 1469 | - | linker |
| Glu 1470 | - | linker |
| Asp 1471 | - | linker |
| Lys 1472 | - | linker |
| Ala 1473 | - | linker |
| Ala 1474 | - | linker |
| Val 1475 | - | linker |
| Glu 1476 | - | linker |
| Arg 1477 | - | linker |
| Ser 1478 | - | linker |
| Lys 1479 | - | linker |
| Met 1480 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asn 1509 | Ser 54 | conflict |
| Ala 1681 | Gly 226 | conflict |
| Ala 1723 | Glu 268 | conflict |
| Lys 1726 | Asn 271 | conflict |
| Asp 1729 | Lys 274 | conflict |
| Lys 1735 | Arg 280 | conflict |
| Asp 1739 | Thr 284 | conflict |
| Thr 1740 | Ile 285 | conflict |
| Ser 1821 | Ala 366 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 185266.9 | |
| All* | Total formula weight | 185266.9 |
