8T7R
Crystal structure of human leukocyte antigen A*0101 in complex with the Fab of alloreactive antibody E07
Summary for 8T7R
| Entry DOI | 10.2210/pdb8t7r/pdb |
| Descriptor | Light chain from antibody JTK191b E07, Fab heavy chain from antibody JTK191b E07, MHC class I antigen (Fragment), ... (5 entities in total) |
| Functional Keywords | human leukocyte antigen, hla, hla class i, antibodies, autoimmunity, fab, alloreactive, immune system |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 50 |
| Total formula weight | 972578.05 |
| Authors | Green, T.J.,Killian Jr, J.T.,Qiu, S.,Macon, K.J.,Yang, G.,King, R.G.,Lund, F.E. (deposition date: 2023-06-21, release date: 2024-12-25, Last modification date: 2026-01-14) |
| Primary citation | Killian Jr., J.T.,King, R.G.,Lucander, A.C.K.,Kizziah, J.L.,Fucile, C.F.,Diaz-Avalos, R.,Qiu, S.,Silva-Sanchez, A.,Mousseau, B.J.,Macon, K.J.,Callahan, A.R.,Yang, G.,Hossain, M.E.,Akther, J.,Good, D.B.,Kelso, S.,Houp, J.A.,Rosenblum, F.,Porrett, P.M.,Ong, S.C.,Kumar, V.,Saphire, E.O.,Kearney, J.F.,Randall, T.D.,Rosenberg, A.F.,Green, T.J.,Lund, F.E. Topography of the HLA-A protein enforces shared and convergent immunodominant B cell and antibody alloresponses in transplant recipients. Immunity, 58:3040-3060.e12, 2025 Cited by PubMed Abstract: Donor-specific antibody responses against human leukocyte antigen (HLA) proteins mismatched between transplant donors and recipients cause allograft loss, yet the structural HLA epitopes targeted by alloreactive B cells and antibodies remain largely unresolved. We profiled the HLA-A01:01-specific B cell response in the transplanted kidney and blood of a recipient undergoing antibody-mediated rejection and identified immunodominant B cell and antibody responses that emerged early in the alloimmune response. These responses were focused on topographically exposed mismatched HLA residues located in the α helices along the peptide-binding groove of HLA-A01:01. We demonstrated that the anti-HLA-A01:01 B cell alloresponse converged and was maintained on this same immunodominant HLA subregion, which comprises only 20% of the HLA molecule, in a diverse group of HLA-A01:01-mismatched transplant recipients. Thus, the B cell and antibody alloresponses appear tightly focused on a topographically defined region on the HLA-A01:01 crown that is conserved across individuals expressing distinct constellations of self-HLA-A. PubMed: 41289995DOI: 10.1016/j.immuni.2025.10.014 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.84 Å) |
Structure validation
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