8R5H

Ubiquitin ligation to neosubstrate by a cullin-RING E3 ligase & Cdc34: NEDD8-CUL2-RBX1-ELOB/C-VHL-MZ1 with trapped UBE2R2~donor UB-BRD4 BD2

Summary for 8R5H

• Entry DOI: 10.2210/pdb8r5h/pdb
• EMDB information: 18207 18230 18915
• Descriptor: Cullin-2, 5-azanylpentan-2-one, (2~{S},4~{R})-1-[(2~{S})-2-[2-[2-[2-[2-[2-[(9~{S})-7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetrazatricyclo[8.3.0.0^{2,6}]trideca-2(6),4,7,10,12-pentaen-9-yl]ethanoylamino]ethoxy]ethoxy]ethoxy]ethanoylamino]-3,3-dimethyl-butanoyl]-~{N}-[[4-(4-methyl-2,3-dihydro-1,3-thiazol-5-yl)phenyl]methyl]-4-oxidanyl-pyrrolidine-2-carboxamide, ... (11 entities in total)
• Functional Keywords: cul2, vhl, elobc, brd4, mz1, protac, ubiquitin, ubiquitin ligase, monoubiquitylation, ligase, nedd8, rbx1
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 192167.28

Authors

Liwocha, J.,Prabu, J.R.,Kleiger, G.,Schulman, B.A. (deposition date: 2023-11-16, release date: 2024-02-21, Last modification date: 2024-04-17)

• Primary citation: Li, J.,Purser, N.,Liwocha, J.,Scott, D.C.,Byers, H.A.,Steigenberger, B.,Hill, S.,Tripathi-Giesgen, I.,Hinkle, T.,Hansen, F.M.,Prabu, J.R.,Radhakrishnan, S.K.,Kirkpatrick, D.S.,Reichermeier, K.M.,Schulman, B.A.,Kleiger, G.; Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting.; Mol.Cell, 84:1304-, 2024; PubMed: 38382526; DOI: 10.1016/j.molcel.2024.01.022

Experimental method

ELECTRON MICROSCOPY (3.44 Å)