8R5H
Ubiquitin ligation to neosubstrate by a cullin-RING E3 ligase & Cdc34: NEDD8-CUL2-RBX1-ELOB/C-VHL-MZ1 with trapped UBE2R2~donor UB-BRD4 BD2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cullin-2 | polymer | 745 | 87098.9 | 1 | UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (human) | CUL-2 |
| 2 | B (R) | E3 ubiquitin-protein ligase RBX1, N-terminally processed | polymer | 104 | 11945.5 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,N-terminally processed |
| 3 | C (C) | Ubiquitin-conjugating enzyme E2 R2 | polymer | 238 | 25853.7 | 1 | UniProt (Q712K3) Pfam (PF00179) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme R2,Ubiquitin carrier protein R2,Ubiquitin-conjugating enzyme E2-CDC34B,Ubiquitin-protein ligase R2 |
| 4 | D (U) | Ubiquitin | polymer | 75 | 8519.8 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (human) | |
| 5 | E (D) | Elongin-C | polymer | 112 | 12485.1 | 1 | UniProt (Q15369) Pfam (PF03931) | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 6 | F (F) | Bromodomain-containing protein 4 | polymer | 115 | 13256.3 | 1 | UniProt (O60885) Pfam (PF00439) | Homo sapiens (human) | Protein HUNK1 |
| 7 | G (G) | Elongin-B | polymer | 118 | 13147.8 | 1 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 8 | H (H) | von Hippel-Lindau disease tumor suppressor | polymer | 160 | 18558.2 | 1 | UniProt (P40337) Pfam (PF01847) Pfam (PF17211) | Homo sapiens (human) | Protein G7,pVHL |
| 9 | I, J, K (R) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 10 | L (C) | 5-azanylpentan-2-one | non-polymer | 101.1 | 1 | Chemie (SY8) | |||
| 11 | M (H) | (2~{S},4~{R})-1-[(2~{S})-2-[2-[2-[2-[2-[2-[(9~{S})-7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetrazatricyclo[8.3.0.0^{2,6}]trideca-2(6),4,7,10,12-pentaen-9-yl]ethanoylamino]ethoxy]ethoxy]ethoxy]ethanoylamino]-3,3-dimethyl-butanoyl]-~{N}-[[4-(4-methyl-2,3-dihydro-1,3-thiazol-5-yl)phenyl]methyl]-4-oxidanyl-pyrrolidine-2-carboxamide | non-polymer | 1004.7 | 1 | Chemie (759) |
Sequence modifications
C: 1 - 192 (UniProt: Q712K3)
C: 1000 - 1045 (PDB: 8R5H)
F: 346 - 460 (UniProt: O60885)
C: 1000 - 1045 (PDB: 8R5H)
F: 346 - 460 (UniProt: O60885)
| PDB | External Database | Details |
|---|---|---|
| Ala 356 | Cys 356 | engineered mutation |
| Ala 357 | Cys 357 | engineered mutation |
| Cys 368 | Lys 368 | engineered mutation |
| Ala 391 | Cys 391 | engineered mutation |
| Ala 429 | Cys 429 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 190865.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1302.0 | |
| All* | Total formula weight | 192167.3 |
