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8R5H

Ubiquitin ligation to neosubstrate by a cullin-RING E3 ligase & Cdc34: NEDD8-CUL2-RBX1-ELOB/C-VHL-MZ1 with trapped UBE2R2~donor UB-BRD4 BD2

Functional Information from GO Data
ChainGOidnamespacecontents
A0000082biological_processG1/S transition of mitotic cell cycle
A0004842molecular_functionubiquitin-protein transferase activity
A0005515molecular_functionprotein binding
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005730cellular_componentnucleolus
A0005829cellular_componentcytosol
A0006511biological_processubiquitin-dependent protein catabolic process
A0010498biological_processproteasomal protein catabolic process
A0016567biological_processprotein ubiquitination
A0019005cellular_componentSCF ubiquitin ligase complex
A0030163biological_processprotein catabolic process
A0030674molecular_functionprotein-macromolecule adaptor activity
A0031146biological_processSCF-dependent proteasomal ubiquitin-dependent protein catabolic process
A0031461cellular_componentcullin-RING ubiquitin ligase complex
A0031462cellular_componentCul2-RING ubiquitin ligase complex
A0031625molecular_functionubiquitin protein ligase binding
A0031981cellular_componentnuclear lumen
A0097193biological_processintrinsic apoptotic signaling pathway
A0140627biological_processubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A0160072molecular_functionubiquitin ligase complex scaffold activity
D0000151cellular_componentubiquitin ligase complex
D0001222molecular_functiontranscription corepressor binding
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005829cellular_componentcytosol
D0006357biological_processregulation of transcription by RNA polymerase II
D0006367biological_processtranscription initiation at RNA polymerase II promoter
D0006511biological_processubiquitin-dependent protein catabolic process
D0016567biological_processprotein ubiquitination
D0030674molecular_functionprotein-macromolecule adaptor activity
D0031462cellular_componentCul2-RING ubiquitin ligase complex
D0031466cellular_componentCul5-RING ubiquitin ligase complex
D0070449cellular_componentelongin complex
D0140958biological_processtarget-directed miRNA degradation
G0000151cellular_componentubiquitin ligase complex
G0001222molecular_functiontranscription corepressor binding
G0005515molecular_functionprotein binding
G0005634cellular_componentnucleus
G0005654cellular_componentnucleoplasm
G0005737cellular_componentcytoplasm
G0005829cellular_componentcytosol
G0006367biological_processtranscription initiation at RNA polymerase II promoter
G0006368biological_processtranscription elongation by RNA polymerase II
G0016567biological_processprotein ubiquitination
G0030891cellular_componentVCB complex
G0031462cellular_componentCul2-RING ubiquitin ligase complex
G0031466cellular_componentCul5-RING ubiquitin ligase complex
G0031625molecular_functionubiquitin protein ligase binding
G0032436biological_processpositive regulation of proteasomal ubiquitin-dependent protein catabolic process
G0065003biological_processprotein-containing complex assembly
G0070449cellular_componentelongin complex
G0140958biological_processtarget-directed miRNA degradation
R0000045biological_processautophagosome assembly
R0000082biological_processG1/S transition of mitotic cell cycle
R0000165biological_processMAPK cascade
R0000209biological_processprotein polyubiquitination
R0000423biological_processmitophagy
R0001837biological_processepithelial to mesenchymal transition
R0004842molecular_functionubiquitin-protein transferase activity
R0005515molecular_functionprotein binding
R0005634cellular_componentnucleus
R0005654cellular_componentnucleoplasm
R0005737cellular_componentcytoplasm
R0005829cellular_componentcytosol
R0006281biological_processDNA repair
R0006283biological_processtranscription-coupled nucleotide-excision repair
R0006366biological_processtranscription by RNA polymerase II
R0006368biological_processtranscription elongation by RNA polymerase II
R0006511biological_processubiquitin-dependent protein catabolic process
R0006513biological_processprotein monoubiquitination
R0006915biological_processapoptotic process
R0006974biological_processDNA damage response
R0006979biological_processresponse to oxidative stress
R0007040biological_processlysosome organization
R0007283biological_processspermatogenesis
R0007346biological_processregulation of mitotic cell cycle
R0008270molecular_functionzinc ion binding
R0008286biological_processinsulin receptor signaling pathway
R0010507biological_processnegative regulation of autophagy
R0010508biological_processpositive regulation of autophagy
R0016567biological_processprotein ubiquitination
R0016740molecular_functiontransferase activity
R0019005cellular_componentSCF ubiquitin ligase complex
R0019221biological_processcytokine-mediated signaling pathway
R0019788molecular_functionNEDD8 transferase activity
R0030163biological_processprotein catabolic process
R0030330biological_processDNA damage response, signal transduction by p53 class mediator
R0030891cellular_componentVCB complex
R0031146biological_processSCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R0031461cellular_componentcullin-RING ubiquitin ligase complex
R0031462cellular_componentCul2-RING ubiquitin ligase complex
R0031463cellular_componentCul3-RING ubiquitin ligase complex
R0031464cellular_componentCul4A-RING E3 ubiquitin ligase complex
R0031465cellular_componentCul4B-RING E3 ubiquitin ligase complex
R0031466cellular_componentCul5-RING ubiquitin ligase complex
R0031467cellular_componentCul7-RING ubiquitin ligase complex
R0031625molecular_functionubiquitin protein ligase binding
R0031669biological_processcellular response to nutrient levels
R0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
R0032436biological_processpositive regulation of proteasomal ubiquitin-dependent protein catabolic process
R0032480biological_processnegative regulation of type I interferon production
R0032481biological_processpositive regulation of type I interferon production
R0032869biological_processcellular response to insulin stimulus
R0034198biological_processcellular response to amino acid starvation
R0034450molecular_functionubiquitin-ubiquitin ligase activity
R0034599biological_processcellular response to oxidative stress
R0034644biological_processcellular response to UV
R0035279biological_processmiRNA-mediated gene silencing by mRNA destabilization
R0038066biological_processp38MAPK cascade
R0038202biological_processTORC1 signaling
R0042110biological_processT cell activation
R0042770biological_processsignal transduction in response to DNA damage
R0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
R0043124biological_processnegative regulation of canonical NF-kappaB signal transduction
R0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
R0043687biological_processpost-translational protein modification
R0044877molecular_functionprotein-containing complex binding
R0045116biological_processprotein neddylation
R0045727biological_processpositive regulation of translation
R0045732biological_processpositive regulation of protein catabolic process
R0045944biological_processpositive regulation of transcription by RNA polymerase II
R0046627biological_processnegative regulation of insulin receptor signaling pathway
R0046872molecular_functionmetal ion binding
R0060090molecular_functionmolecular adaptor activity
R0060337biological_processtype I interferon-mediated signaling pathway
R0061629molecular_functionRNA polymerase II-specific DNA-binding transcription factor binding
R0061630molecular_functionubiquitin protein ligase activity
R0061663molecular_functionNEDD8 ligase activity
R0062197biological_processcellular response to chemical stress
R0070294biological_processrenal sodium ion absorption
R0070936biological_processprotein K48-linked ubiquitination
R0071230biological_processcellular response to amino acid stimulus
R0080008cellular_componentCul4-RING E3 ubiquitin ligase complex
R0090090biological_processnegative regulation of canonical Wnt signaling pathway
R0090734cellular_componentsite of DNA damage
R0097510biological_processbase-excision repair, AP site formation via deaminated base removal
R0097602molecular_functioncullin family protein binding
R0140627biological_processubiquitin-dependent protein catabolic process via the C-end degron rule pathway
R0160240biological_processRNA polymerase II transcription initiation surveillance
R1900076biological_processregulation of cellular response to insulin stimulus
R1901525biological_processnegative regulation of mitophagy
R1901797biological_processnegative regulation of signal transduction by p53 class mediator
R1902499biological_processpositive regulation of protein autoubiquitination
R1902883biological_processnegative regulation of response to oxidative stress
R1904037biological_processpositive regulation of epithelial cell apoptotic process
R1904262biological_processnegative regulation of TORC1 signaling
R1904263biological_processpositive regulation of TORC1 signaling
R2000059biological_processnegative regulation of ubiquitin-dependent protein catabolic process
Functional Information from PROSITE/UniProt
site_idPS00183
Number of Residues16
DetailsUBC_1 Ubiquitin-conjugating (UBC) active site signature. WHPNIyen.GdVCIsiL
ChainResidueDetails
CTRP82-LEU97

site_idPS00299
Number of Residues26
DetailsUBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD
ChainResidueDetails
ULYS27-ASP52

site_idPS01256
Number of Residues28
DetailsCULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA
ChainResidueDetails
AILE718-ALA745

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsMOD_RES: N-acetylmethionine => ECO:0000269|Ref.6, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22814378
ChainResidueDetails
GMET1
UARG72

site_idSWS_FT_FI2
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P62869
ChainResidueDetails
GTHR84
RCYS45
RCYS75
RHIS77
RHIS80
RCYS94
RASP97

site_idSWS_FT_FI3
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P62869
ChainResidueDetails
GSER108
GSER111
RCYS68
RHIS82

site_idSWS_FT_FI4
Number of Residues1
DetailsMOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457
ChainResidueDetails
UTHR66

site_idSWS_FT_FI5
Number of Residues1
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603
ChainResidueDetails
ULYS6

site_idSWS_FT_FI6
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106
ChainResidueDetails
ULYS63

site_idSWS_FT_FI7
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144
ChainResidueDetails
ULYS11
ULYS48

site_idSWS_FT_FI8
Number of Residues1
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860
ChainResidueDetails
ULYS27

site_idSWS_FT_FI9
Number of Residues1
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127
ChainResidueDetails
ULYS29

site_idSWS_FT_FI10
Number of Residues1
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577
ChainResidueDetails
ULYS33

237992

PDB entries from 2025-06-25

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