8QH4
Crystal structure of reduced respiratory Complex I subunits NuoEF from Aquifex aeolicus bound to oxidized 3-acetylpyridine adenine dinucleotide
Summary for 8QH4
| Entry DOI | 10.2210/pdb8qh4/pdb |
| Related | 8QG1 |
| Descriptor | NADH-quinone oxidoreductase subunit E, POTASSIUM ION, NADH-quinone oxidoreductase subunit F, ... (11 entities in total) |
| Functional Keywords | complex i, respiratory chain, 3-acetylpyridine dinucleotide, nadh-binding, oxidoreductase |
| Biological source | Aquifex aeolicus VF5 More |
| Total number of polymer chains | 4 |
| Total formula weight | 138570.00 |
| Authors | Wohlwend, D.,Friedrich, T. (deposition date: 2023-09-06, release date: 2024-04-03, Last modification date: 2024-06-19) |
| Primary citation | Wohlwend, D.,Merono, L.,Bucka, S.,Ritter, K.,Jessen, H.J.,Friedrich, T. Structures of 3-acetylpyridine adenine dinucleotide and ADP-ribose bound to the electron input module of respiratory complex I. Structure, 32:715-, 2024 Cited by PubMed Abstract: Energy-converting NADH:ubiquinone oxidoreductase, respiratory complex I, is a major enzyme of energy metabolism that couples NADH oxidation and ubiquinone reduction with proton translocation. The NADH oxidation site features different enzymatic activities with various nucleotides. While the kinetics of these reactions are well described, only binding of NAD and NADH have been structurally characterized. Here, we report the structures of the electron input module of Aquifex aeolicus complex I with bound ADP-ribose and 3-acetylpyridine adenine dinucleotides at resolutions better than 2.0 Å. ADP-ribose acts as inhibitor by blocking the "ADP-handle" motif essential for nucleotide binding. The pyridine group of APADH is minimally offset from flavin, which could contribute to its poorer suitability as substrate. A comparison with other nucleotide co-structures surprisingly shows that the adenine ribose and the pyrophosphate moiety contribute most to nucleotide binding, thus all adenine dinucleotides share core binding modes to the unique Rossmann-fold in complex I. PubMed: 38503292DOI: 10.1016/j.str.2024.02.013 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.96 Å) |
Structure validation
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