8QH4

Crystal structure of reduced respiratory Complex I subunits NuoEF from Aquifex aeolicus bound to oxidized 3-acetylpyridine adenine dinucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	63.527, 116.619, 190.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.153 - 1.960
Rwork	0.192
R-free	0.22440
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	1.152
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.9)
Phasing software	REFMAC (5.8.0352)
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.153	1.990
High resolution limit [Å]	1.960	1.960
Rpim	0.043	0.318
Number of reflections	102156	4996
<I/σ(I)>	14.4	2.6
Completeness [%]	100.0	100
Redundancy	13.2	13.4
CC(1/2)	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	trisodium citrate, ammonium sulfate, sodium chloride, Tris, BisTris