Summary for 8OWT
| Entry DOI | 10.2210/pdb8owt/pdb |
| Descriptor | Nanobody A8, Spike protein S1, Nanobody H3, ... (6 entities in total) |
| Functional Keywords | nanobody, complex, receptor binding domain, sars-cov-2, viral protein |
| Biological source | Lama glama More |
| Total number of polymer chains | 6 |
| Total formula weight | 107302.55 |
| Authors | Mikolajek, H.,Naismith, J.H.,Owens, R.J. (deposition date: 2023-04-28, release date: 2024-05-08, Last modification date: 2024-11-06) |
| Primary citation | Cornish, K.,Huo, J.,Jones, L.,Sharma, P.,Thrush, J.W.,Abdelkarim, S.,Kipar, A.,Ramadurai, S.,Weckener, M.,Mikolajek, H.,Liu, S.,Buckle, I.,Bentley, E.,Kirby, A.,Han, X.,Laidlaw, S.M.,Hill, M.,Eyssen, L.,Norman, C.,Le Bas, A.,Clarke, J.,James, W.,Stewart, J.P.,Carroll, M.,Naismith, J.H.,Owens, R.J. Structural and functional characterization of nanobodies that neutralize Omicron variants of SARS-CoV-2. Open Biology, 14:230252-230252, 2024 Cited by PubMed Abstract: The Omicron strains of SARS-CoV-2 pose a significant challenge to the development of effective antibody-based treatments as immune evasion has compromised most available immune therapeutics. Therefore, in the 'arms race' with the virus, there is a continuing need to identify new biologics for the prevention or treatment of SARS-CoV-2 infections. Here, we report the isolation of nanobodies that bind to the Omicron BA.1 spike protein by screening nanobody phage display libraries previously generated from llamas immunized with either the Wuhan or Beta spike proteins. The structure and binding properties of three of these nanobodies (A8, H6 and B5-5) have been characterized in detail providing insight into their binding epitopes on the Omicron spike protein. Trimeric versions of H6 and B5-5 neutralized the SARS-CoV-2 variant of concern BA.5 both and in the hamster model of COVID-19 following nasal administration. Thus, either alone or in combination could serve as starting points for the development of new anti-viral immunotherapeutics. PubMed: 38835241DOI: 10.1098/rsob.230252 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.37 Å) |
Structure validation
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