8KDE

Cryo-EM structure of an intermediate-state complex during the process of photosystem II repair

Summary for 8KDE

• Entry DOI: 10.2210/pdb8kde/pdb
• EMDB information: 37133
• Descriptor: Photosystem II CP47 reaction center protein, Photosystem II reaction center protein T, Photosystem II reaction center protein Ycf12, ... (30 entities in total)
• Functional Keywords: monomeric photosystem ii, psii repair, photosynthesis
• Biological source: Chlamydomonas reinhardtii; More
• Total number of polymer chains: 18
• Total formula weight: 351841.13

Authors

Li, A.,Wang, Y.,Liu, Z. (deposition date: 2023-08-09, release date: 2024-06-19, Last modification date: 2024-06-26)

Primary citation

Li, A.,You, T.,Pang, X.,Wang, Y.,Tian, L.,Li, X.,Liu, Z.
Structural basis for an early stage of the photosystem II repair cycle in Chlamydomonas reinhardtii.
Nat Commun, 15:5211-5211, 2024

PubMed Abstract: Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs to be restored through a process known as the PSII repair cycle. The detailed molecular mechanism underlying the PSII repair process remains mostly elusive. Here, we report biochemical and structural features of a PSII-repair intermediate complex, likely arrested at an early stage of the PSII repair process in the green alga Chlamydomonas reinhardtii. The complex contains three protein factors associated with a damaged PSII core, namely Thylakoid Enriched Factor 14 (TEF14), Photosystem II Repair Factor 1 (PRF1), and Photosystem II Repair Factor 2 (PRF2). TEF14, PRF1 and PRF2 may facilitate the release of the manganese-stabilizing protein PsbO, disassembly of peripheral light-harvesting complexes from PSII and blockage of the Q site, respectively. Moreover, an α-tocopherol quinone molecule is located adjacent to the heme group of cytochrome b, potentially fulfilling a photoprotective role by preventing the generation of reactive oxygen species.

PubMed: 38890314
DOI: 10.1038/s41467-024-49532-2

Experimental method

ELECTRON MICROSCOPY (2.6 Å)