8HNV

CryoEM structure of HpaCas9-sgRNA-dsDNA in the presence of AcrIIC4

Summary for 8HNV

Entry DOI	10.2210/pdb8hnv/pdb
EMDB information	34919
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA, target strand, ... (5 entities in total)
Functional Keywords	cas9, cleavage inhibition, antimicrobial protein, hydrolase-rna-antimicrobial protein complex
Biological source	Haemophilus parainfluenzae More
Total number of polymer chains	5
Total formula weight	194170.20
Authors	Sun, W.,Cheng, Z.,Wang, J.,Yang, X.,Wang, Y. (deposition date: 2022-12-08, release date: 2023-07-19, Last modification date: 2023-08-02)
Primary citation	Sun, W.,Cheng, Z.,Wang, J.,Yang, J.,Li, X.,Wang, J.,Chen, M.,Yang, X.,Sheng, G.,Lou, J.,Wang, Y. AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation. Proc.Natl.Acad.Sci.USA, 120:e2303675120-e2303675120, 2023 Cited by PubMed: 37494395 DOI: 10.1073/pnas.2303675120 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.1 Å)

Structure validation