8HNV
CryoEM structure of HpaCas9-sgRNA-dsDNA in the presence of AcrIIC4
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR-associated endonuclease Cas9 | polymer | 1055 | 121605.7 | 1 | UniProt (F0ET08) Pfam (PF13395) Pfam (PF18541) In PDB | Haemophilus parainfluenzae | |
| 2 | B | sgRNA | polymer | 128 | 40860.1 | 1 | synthetic construct | ||
| 3 | C | target strand | polymer | 35 | 10865.0 | 1 | Haemophilus parainfluenzae | ||
| 4 | D | non-target strand | polymer | 35 | 10767.0 | 1 | Haemophilus parainfluenzae | ||
| 5 | E | anti-CRISPR protein AcrIIC4 | polymer | 89 | 10072.4 | 1 | UniProt (by SIFTS) (A0A377JKY9) In PDB | Haemophilus parainfluenzae |
Sequence modifications
A: 1 - 1054 (UniProt: F0ET08)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 13 | Asp 13 | engineered mutation |
| Ala 581 | His 581 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 194170.2 | |
| All* | Total formula weight | 194170.2 |
