8FYD

Cryo-EM structure of Cas1:Cas2-DEDDh:half-site integration complex bent CRISPR repeat conformation

Summary for 8FYD

Entry DOI	10.2210/pdb8fyd/pdb
EMDB information	29561 29562 29563 29564 29565
Descriptor	DNA (49-MER), Cas2-DEDDh, Cas1, ... (6 entities in total)
Functional Keywords	crispr, integrase, crispr adaptation module, pam, prespacer, exonuclease, dna binding protein-dna complex, enzyme, ribonucleoprotein, dna binding protein/dna
Biological source	Megasphaera More
Total number of polymer chains	10
Total formula weight	272790.72
Authors	Skopintsev, P.,Tuck, O.T.,Soczek, K.M.,Doudna, J. (deposition date: 2023-01-25, release date: 2023-05-03, Last modification date: 2023-07-05)
Primary citation	Wang, J.Y.,Tuck, O.T.,Skopintsev, P.,Soczek, K.M.,Li, G.,Al-Shayeb, B.,Zhou, J.,Doudna, J.A. Genome expansion by a CRISPR trimmer-integrase. Nature, 618:855-861, 2023 Cited by PubMed: 37316664 DOI: 10.1038/s41586-023-06178-2 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.9 Å)

Structure validation