8E1Z

Crystal structure of Plasmodium falciparum ookinete surface antigen Pfs28

Summary for 8E1Z

• Entry DOI: 10.2210/pdb8e1z/pdb
• Descriptor: Ookinete surface protein Pfs28, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: transmission blocking vaccine, cell adhesion
• Biological source: Plasmodium falciparum (malaria parasite P. falciparum)
• Total number of polymer chains: 2
• Total formula weight: 40803.31

Authors

Shukla, N.,Tang, W.K.,Tolia, N.H. (deposition date: 2022-08-12, release date: 2022-11-30, Last modification date: 2024-10-16)

Primary citation

Shukla, N.,Tang, W.K.,Tolia, N.H.
Structural analysis of Plasmodium falciparum ookinete surface antigen Pfs28 relevant for malaria vaccine design.
Sci Rep, 12:19556-19556, 2022

PubMed Abstract: Pfs28 is a Plasmodium falciparum malaria transmission-blocking vaccine candidate that is anchored to the parasite surface through a C-terminal glycosylphosphatidylinositol (GPI) moiety, and plays a role in parasite survival in the mosquito midgut. Pfs28 contains epidermal growth factor (EGF)-like domains and is part of a family of sexual stage malaria proteins that includes the related vaccine antigen Pfs25. The lack of structural definition of Pfs28 and the immune response to this candidate has limited further malaria vaccine development for this antigen. Here, we present the crystal structure of Pfs28, examine its conservation with P. vivax Pvs28, and evaluate the cross-reactivity of Pfs28 to antibodies that recognize Pfs25. Pfs28 is comprised of four EGF-like domains stabilized by ten disulfide bridges with an overall architecture that highly resembles Pfs25. Despite the high sequence and structural similarity between these antigens, no cross reactivity of Pfs28 to anti-Pfs25 monoclonal antibodies could be demonstrated.

PubMed: 36379968
DOI: 10.1038/s41598-022-24054-3

Experimental method

X-RAY DIFFRACTION (2.3 Å)