8E1Z

Crystal structure of Plasmodium falciparum ookinete surface antigen Pfs28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	35.730, 74.180, 69.820
Unit cell angles	90.00, 105.17, 90.00

Refinement procedure

Resolution	34.590 - 2.300
R-factor	0.2389
Rwork	0.235
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q8IJ96-F1
RMSD bond length	0.003
RMSD bond angle	0.567
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.590	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.118	0.676
Rmeas	0.141	0.803
Rpim	0.076	0.429
Number of reflections	15434	1579
<I/σ(I)>	7.02	1.98
Completeness [%]	97.7	98.5
Redundancy	3.4	3.4
CC(1/2)	0.991	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.5M ammonium sulfate and 30% PEG 4000