8DMK

Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies

Summary for 8DMK

• Entry DOI: 10.2210/pdb8dmk/pdb
• EMDB information: 27542
• Descriptor: Laminin subunit alpha-1, Laminin subunit beta-1, Laminin subunit gamma-1, ... (5 entities in total)
• Functional Keywords: laminin, complex, basement membrane, structural protein
• Biological source: Mus musculus (mouse); More
• Total number of polymer chains: 3
• Total formula weight: 105058.05

Authors

Kulczyk, A.W. (deposition date: 2022-07-08, release date: 2023-02-01, Last modification date: 2024-11-06)

Primary citation

Kulczyk, A.W.,McKee, K.K.,Zhang, X.,Bizukojc, I.,Yu, Y.Q.,Yurchenco, P.D.
Cryo-EM reveals the molecular basis oflaminin polymerization and LN-lamininopathies.
Nat Commun, 14:317-317, 2023

PubMed Abstract: Laminin polymerization is the major step in basement membranes assembly. Its failures cause laminin N-terminal domain lamininopathies including Pierson syndrome. We have employed cryo-electron microscopy to determine a 3.7 Å structure of the trimeric laminin polymer node containing α1, β1 and γ1 subunits. The structure reveals the molecular basis of calcium-dependent formation of laminin lattice, and provides insights into polymerization defects manifesting in human disease.

PubMed: 36658135
DOI: 10.1038/s41467-023-36077-z

Experimental method

ELECTRON MICROSCOPY (3.7 Å)