Summary for 8BOT
| Entry DOI | 10.2210/pdb8bot/pdb |
| EMDB information | 16145 |
| Descriptor | DNA repair protein XRCC4, DNA (24-MER), DNA ligase 4, ... (10 entities in total) |
| Functional Keywords | nhej, dna-pk, dna-pkcs, ku70, ku80, xlf, dna repair, dna binding protein |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 25 |
| Total formula weight | 2411092.49 |
| Authors | Hardwick, S.W.,Chaplin, A.K. (deposition date: 2022-11-15, release date: 2023-06-28, Last modification date: 2023-08-16) |
| Primary citation | Hardwick, S.W.,Stavridi, A.K.,Chirgadze, D.Y.,De Oliveira, T.M.,Charbonnier, J.B.,Ropars, V.,Meek, K.,Blundell, T.L.,Chaplin, A.K. Cryo-EM structure of a DNA-PK trimer: higher order oligomerisation in NHEJ. Structure, 31:895-, 2023 Cited by PubMed Abstract: The ability of humans to maintain the integrity of the genome is imperative for cellular survival. DNA double-strand breaks (DSBs) are considered the most critical type of DNA lesion, which can ultimately lead to diseases including cancer. Non-homologous end joining (NHEJ) is one of two core mechanisms utilized to repair DSBs. DNA-PK is a key component in this process and has recently been shown to form alternate long-range synaptic dimers. This has led to the proposal that these complexes can be formed before transitioning to a short-range synaptic complex. Here we present cryo-EM data representing an NHEJ supercomplex consisting of a trimer of DNA-PK in complex with XLF, XRCC4, and DNA Ligase IV. This trimer represents a complex of both long-range synaptic dimers. We discuss the potential role of the trimeric structure, and possible higher order oligomers, as structural intermediates in the NHEJ mechanism, or as functional DNA repair centers. PubMed: 37311458DOI: 10.1016/j.str.2023.05.013 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.76 Å) |
Structure validation
Download full validation report
