8BAH

Human Mre11-Nbs1 complex

8BAH の概要

• エントリーDOI: 10.2210/pdb8bah/pdb
• EMDBエントリー: 15948
• 分子名称: Double-strand break repair protein MRE11, Nibrin, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: dna repair, complex, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 253310.04

構造登録者

Bartho, J.D.,Rotheneder, M.,Stakyte, K.,Lammens, K.,Hopfner, K.P. (登録日: 2022-10-11, 公開日: 2023-01-11, 最終更新日: 2023-12-13)

主引用文献

Rotheneder, M.,Stakyte, K.,van de Logt, E.,Bartho, J.D.,Lammens, K.,Fan, Y.,Alt, A.,Kessler, B.,Jung, C.,Roos, W.P.,Steigenberger, B.,Hopfner, K.P.
Cryo-EM structure of the Mre11-Rad50-Nbs1 complex reveals the molecular mechanism of scaffolding functions.
Mol.Cell, 83:167-185.e9, 2023

PubMed Abstract: The DNA double-strand break repair complex Mre11-Rad50-Nbs1 (MRN) detects and nucleolytically processes DNA ends, activates the ATM kinase, and tethers DNA at break sites. How MRN can act both as nuclease and scaffold protein is not well understood. The cryo-EM structure of MRN from Chaetomium thermophilum reveals a 2:2:1 complex with a single Nbs1 wrapping around the autoinhibited Mre11 nuclease dimer. MRN has two DNA-binding modes, one ATP-dependent mode for loading onto DNA ends and one ATP-independent mode through Mre11's C terminus, suggesting how it may interact with DSBs and intact DNA. MRNs two 60-nm-long coiled-coil domains form a linear rod structure, the apex of which is assembled by the two joined zinc-hook motifs. Apices from two MRN complexes can further dimerize, forming 120-nm spanning MRN-MRN structures. Our results illustrate the architecture of MRN and suggest how it mechanistically integrates catalytic and tethering functions.

PubMed: 36577401
DOI: 10.1016/j.molcel.2022.12.003

実験手法

ELECTRON MICROSCOPY (4.13 Å)