8AXA

Cryo-EM structure of shCas12k-sgRNA-dsDNA ternary complex (type V-K CRISPR-associated transposon)

Summary for 8AXA

• Entry DOI: 10.2210/pdb8axa/pdb
• Related: 8AXB
• EMDB information: 15697 15698 19075 19282 19283 19284 19286
• Descriptor: Cas12k, sgRNA, DNA target strand, ... (5 entities in total)
• Functional Keywords: crispr, complex, transposition, dna binding protein
• Biological source: Scytonema hofmannii; More
• Total number of polymer chains: 4
• Total formula weight: 192631.59

Authors

Tenjo-Castano, F.,Sofos, N.,Stella, S.,Fuglsang, A.,Pape, T.,Mesa, P.,Stutzke, L.S.,Temperini, P.,Montoya, G. (deposition date: 2022-08-31, release date: 2024-04-10, Last modification date: 2024-07-03)

• Primary citation: Tenjo-Castano, F.,Sofos, N.,Stutzke, L.S.,Temperini, P.,Fuglsang, A.,Pape, T.,Mesa, P.,Montoya, G.; Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.; Mol.Cell, 84:2353-2367.e5, 2024; PubMed: 38834066; DOI: 10.1016/j.molcel.2024.05.005

Experimental method

ELECTRON MICROSCOPY (2.96 Å)