8AXA
Cryo-EM structure of shCas12k-sgRNA-dsDNA ternary complex (type V-K CRISPR-associated transposon)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cas12k | polymer | 651 | 74738.3 | 1 | UniProt (A0A8M0FGU0) UniProt (by SIFTS) (A0A8X6EH11) | Scytonema hofmannii | |
| 2 | B (B) | sgRNA | polymer | 261 | 84006.5 | 1 | Scytonema hofmannii | ||
| 3 | C (C) | DNA target strand | polymer | 55 | 16858.9 | 1 | Scytonema hofmannii | ||
| 4 | D (D) | DNA non-target strand | polymer | 55 | 17028.0 | 1 | Scytonema hofmannii | ||
| 5 | E, F (A, B) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A: 1 - 639 (UniProt: A0A8M0FGU0)
| PDB | External Database | Details |
|---|---|---|
| Gly 640 | - | expression tag |
| Ser 641 | - | expression tag |
| Glu 642 | - | expression tag |
| Phe 643 | - | expression tag |
| Glu 644 | - | expression tag |
| Leu 645 | - | expression tag |
| Glu 646 | - | expression tag |
| Asn 647 | - | expression tag |
| Leu 648 | - | expression tag |
| Tyr 649 | - | expression tag |
| Phe 650 | - | expression tag |
| Gln 651 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 192631.6 | |
| All* | Total formula weight | 192631.6 |
