7ZM2

Crystal structure of HsaD from Mycobacterium tuberculosis in complex with Cyclophostin-like inhibitor CyC8b

7ZM2 の概要

• エントリーDOI: 10.2210/pdb7zm2/pdb
• 関連するPDBエントリー: 7ZJT 7ZM1
• 分子名称: 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase, methoxy-[(3~{R})-3-[(2~{R})-1-methoxy-1,3-bis(oxidanylidene)butan-2-yl]pentadecyl]phosphinic acid, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: hsad, m. tuberculosis, cholesterol, inhibitor, hydrolase
• 由来する生物種: Mycobacterium tuberculosis H37Rv
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67294.54

構造登録者

Barelier, S.,Roig-Zamboni, V.,Cavalier, J.F.,Sulzenbacher, G. (登録日: 2022-04-19, 公開日: 2022-09-28, 最終更新日: 2024-11-20)

主引用文献

Barelier, S.,Avellan, R.,Gnawali, G.R.,Fourquet, P.,Roig-Zamboni, V.,Poncin, I.,Point, V.,Bourne, Y.,Audebert, S.,Camoin, L.,Spilling, C.D.,Canaan, S.,Cavalier, J.F.,Sulzenbacher, G.
Direct capture, inhibition and crystal structure of HsaD (Rv3569c) from M. tuberculosis.
Febs J., 290:1563-1582, 2023

PubMed Abstract: A hallmark of Mycobacterium tuberculosis (M. tb), the aetiologic agent of tuberculosis, is its ability to metabolise host-derived lipids. However, the enzymes and mechanisms underlying such metabolism are still largely unknown. We previously reported that the Cyclophostin & Cyclipostins (CyC) analogues, a new family of potent antimycobacterial molecules, react specifically and covalently with (Ser/Cys)-based enzymes mostly involved in bacterial lipid metabolism. Here, we report the synthesis of new CyC alkyne-containing inhibitors (CyC ) and their use for the direct fishing of target proteins in M. tb culture via bio-orthogonal click-chemistry activity-based protein profiling (CC-ABPP). This approach led to the capture and identification of a variety of enzymes, and many of them involved in lipid or steroid metabolisms. One of the captured enzymes, HsaD (Rv3569c), is required for the survival of M. tb within macrophages and is thus a potential therapeutic target. This prompted us to further explore and validate, through a combination of biochemical and structural approaches, the specificity of HsaD inhibition by the CyC analogues. We confirmed that the CyC bind covalently to the catalytic Ser residue, leading to a total loss of enzyme activity. These data were supported by the X-ray structures of four HsaD-CyC complexes, obtained at resolutions between 1.6 and 2.6 Å. The identification of mycobacterial enzymes directly captured by the CyC probes through CC-ABPP paves the way to better understand and potentially target key players at crucial stages of the bacilli life cycle.

PubMed: 36197115
DOI: 10.1111/febs.16645

実験手法

X-RAY DIFFRACTION (2.2 Å)