7YYN

Mammalian Dicer in the dicing state with pre-miR-15a substrate

Summary for 7YYN

• Entry DOI: 10.2210/pdb7yyn/pdb
• EMDB information: 14384
• Descriptor: 59-nt precursor of miR-15a, Isoform 2 of Endoribonuclease Dicer (2 entities in total)
• Functional Keywords: endoribonuclease, dsrna, complex, gene silencing, post-transcriptional, catalytic complex, cytoplasm, rna binding protein
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 219058.22

Authors

Zanova, M.,Zapletal, D.,Kubicek, K.,Stefl, R.,Pinkas, M.,Novacek, J. (deposition date: 2022-02-18, release date: 2022-11-16, Last modification date: 2024-07-17)

Primary citation

Zapletal, D.,Taborska, E.,Pasulka, J.,Malik, R.,Kubicek, K.,Zanova, M.,Much, C.,Sebesta, M.,Buccheri, V.,Horvat, F.,Jenickova, I.,Prochazkova, M.,Prochazka, J.,Pinkas, M.,Novacek, J.,Joseph, D.F.,Sedlacek, R.,Bernecky, C.,O'Carroll, D.,Stefl, R.,Svoboda, P.
Structural and functional basis of mammalian microRNA biogenesis by Dicer.
Mol.Cell, 82:4064-4079.e13, 2022

PubMed Abstract: MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucleases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is specifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer's DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the complete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicer•-miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleavage-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways.

PubMed: 36332606
DOI: 10.1016/j.molcel.2022.10.010

Experimental method

ELECTRON MICROSCOPY (6.21 Å)