7YYN

Mammalian Dicer in the dicing state with pre-miR-15a substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	59-nt precursor of miR-15a	polymer	59	19017.3	1	GenBank (66773608)	Mus musculus
2	B (A)	Isoform 2 of Endoribonuclease Dicer	polymer	1765	200040.9	1	UniProt (Q8R418) Pfam (PF20930) Pfam (PF00271) Pfam (PF03368) Pfam (PF20931) Pfam (PF02170) Pfam (PF00636) Pfam (PF20932)	Mus musculus (house mouse)	Double-strand-specific ribonuclease mDCR-1

Sequence modifications

A: 2 - 1678 (UniProt: Q8R418)

PDB	External Database	Details
Met -51	-	initiating methionine
Val -50	-	expression tag
Trp -49	-	expression tag
Ser -48	-	expression tag
His -47	-	expression tag
Pro -46	-	expression tag
Gln -45	-	expression tag
Phe -44	-	expression tag
Glu -43	-	expression tag
Lys -42	-	expression tag
Gly -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
Gly -37	-	expression tag
Gly -36	-	expression tag
Gly -35	-	expression tag
Ser -34	-	expression tag
Gly -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ala -30	-	expression tag
Trp -29	-	expression tag
Ser -28	-	expression tag
His -27	-	expression tag
Pro -26	-	expression tag
Gln -25	-	expression tag
Phe -24	-	expression tag
Glu -23	-	expression tag
Lys -22	-	expression tag
Gly -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Pro -18	-	expression tag
Tyr -17	-	expression tag
Asp -16	-	expression tag
Val -15	-	expression tag
Pro -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Ala -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Val 0	-	expression tag
Asp 1	-	expression tag
Ser 872	Thr 872	conflict
Ala 1322	Glu 1322	engineered mutation
Ser 1381	Ala 1381	conflict
Ala 1569	Glu 1569	engineered mutation
Gly 1679	-	expression tag
Arg 1680	-	expression tag
Gly 1681	-	expression tag
Glu 1682	-	expression tag
Asn 1683	-	expression tag
Leu 1684	-	expression tag
Tyr 1685	-	expression tag
Phe 1686	-	expression tag
Gln 1687	-	expression tag
Gly 1688	-	expression tag
Ala 1689	-	expression tag
Ser 1690	-	expression tag
Asp 1691	-	expression tag
Tyr 1692	-	expression tag
Lys 1693	-	expression tag
Asp 1694	-	expression tag
His 1695	-	expression tag
Asp 1696	-	expression tag
Gly 1697	-	expression tag
Asp 1698	-	expression tag
Tyr 1699	-	expression tag
Lys 1700	-	expression tag
Asp 1701	-	expression tag
His 1702	-	expression tag
Asp 1703	-	expression tag
Gly 1704	-	expression tag
Ser 1705	-	expression tag
His 1706	-	expression tag
His 1707	-	expression tag
His 1708	-	expression tag
His 1709	-	expression tag
His 1710	-	expression tag
His 1711	-	expression tag
His 1712	-	expression tag
His 1713	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	219058.2
All*	Total formula weight	219058.2

*Water molecules are not included.