7W02

Cryo-EM structure of ATP-bound ABCA3

Summary for 7W02

• Entry DOI: 10.2210/pdb7w02/pdb
• EMDB information: 32233 32234
• Descriptor: Phospholipid-transporting ATPase ABCA3, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: abc transporter, membrane protein, translocase
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 198017.08

Authors

Xie, T.,Zhang, Z.K.,Yue, J.,Gong, X. (deposition date: 2021-11-17, release date: 2022-04-20, Last modification date: 2025-06-25)

Primary citation

Xie, T.,Zhang, Z.,Yue, J.,Fang, Q.,Gong, X.
Cryo-EM structures of the human surfactant lipid transporter ABCA3.
Sci Adv, 8:eabn3727-eabn3727, 2022

PubMed Abstract: The adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter ABCA3 plays a critical role in pulmonary surfactant biogenesis. Mutations in human ABCA3 have been recognized as the most frequent causes of inherited surfactant dysfunction disorders. Despite two decades of research, in vitro biochemical and structural studies of ABCA3 are still lacking. Here, we report the cryo-EM structures of human ABCA3 in two distinct conformations, both at resolution of 3.3 Å. In the absence of ATP, ABCA3 adopts a "lateral-opening" conformation with the lateral surfaces of transmembrane domains (TMDs) exposed to the membrane and features two positively charged cavities within the TMDs as potential substrate binding sites. ATP binding induces pronounced conformational changes, resulting in the collapse of the potential substrate binding cavities. Our results help to rationalize the disease-causing mutations in human ABCA3 and suggest a conserved "lateral access and extrusion" mechanism for both lipid export and import mediated by ABCA transporters.

PubMed: 35394827
DOI: 10.1126/sciadv.abn3727

Experimental method

ELECTRON MICROSCOPY (3.3 Å)