7V59
Cryo-EM structure of spyCas9-sgRNA-DNA dimer
Summary for 7V59
Entry DOI | 10.2210/pdb7v59/pdb |
EMDB information | 31721 |
Descriptor | CRISPR-associated endonuclease Cas9/Csn1, RNA (115-MER), DNA (49-MER) (3 entities in total) |
Functional Keywords | complex, rna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 6 |
Total formula weight | 420577.91 |
Authors | |
Primary citation | Yang, M.,Sun, R.,Deng, P.,Yang, Y.,Wang, W.,Liu, J.G.,Chen, C. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem Sci, 12:12776-12784, 2021 Cited by PubMed: 34703564DOI: 10.1039/d1sc02633j PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (5.26 Å) |
Structure validation
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