7TQL

CryoEM structure of the human 40S small ribosomal subunit in complex with translation initiation factors eIF1A and eIF5B.

7TQL の概要

• エントリーDOI: 10.2210/pdb7tql/pdb
• EMDBエントリー: 26067
• 分子名称: Eukaryotic translation initiation factor 5B, 40S ribosomal protein S7, 40S ribosomal protein S8, ... (40 entities in total)
• 機能のキーワード: eif5b, translation, initiation, eif1a, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 38
• 化学式量合計: 1234819.23

構造登録者

Lapointe, C.P.,Grosely, R.,Sokabe, M.,Alvarado, C.,Wang, J.,Montabana, E.,Villa, N.,Shin, B.,Dever, T.,Fraser, C.,Fernandez, I.S.,Puglisi, J.D. (登録日: 2022-01-26, 公開日: 2022-04-27, 最終更新日: 2024-06-12)

主引用文献

Lapointe, C.P.,Grosely, R.,Sokabe, M.,Alvarado, C.,Wang, J.,Montabana, E.,Villa, N.,Shin, B.S.,Dever, T.E.,Fraser, C.S.,Fernandez, I.S.,Puglisi, J.D.
eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.
Nature, 607:185-190, 2022

PubMed Abstract: Translation initiation defines the identity and quantity of a synthesized protein. The process is dysregulated in many human diseases. A key commitment step is when the ribosomal subunits join at a translation start site on a messenger RNA to form a functional ribosome. Here, we combined single-molecule spectroscopy and structural methods using an in vitro reconstituted system to examine how the human ribosomal subunits join. Single-molecule fluorescence revealed when the universally conserved eukaryotic initiation factors eIF1A and eIF5B associate with and depart from initiation complexes. Guided by single-molecule dynamics, we visualized initiation complexes that contained both eIF1A and eIF5B using single-particle cryo-electron microscopy. The resulting structure revealed how eukaryote-specific contacts between the two proteins remodel the initiation complex to orient the initiator aminoacyl-tRNA in a conformation compatible with ribosomal subunit joining. Collectively, our findings provide a quantitative and architectural framework for the molecular choreography orchestrated by eIF1A and eIF5B during translation initiation in humans.

PubMed: 35732735
DOI: 10.1038/s41586-022-04858-z

実験手法

ELECTRON MICROSCOPY (3.2 Å)