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7P0R

Crystal structure of L-Trp/Indoleamine 2,3-dioxygenase (hIDO1) complex with the JK-loop refined in the intermediate conformation

Summary for 7P0R
Entry DOI10.2210/pdb7p0r/pdb
DescriptorIndoleamine 2,3-dioxygenase 1, PROTOPORPHYRIN IX CONTAINING FE, GLYCEROL, ... (7 entities in total)
Functional Keywordsl-trp metabolism, hemoprotein, dynamics loop, oxidoreductase
Biological sourceHomo sapiens (Human)
Total number of polymer chains4
Total formula weight186513.08
Authors
Mirgaux, M.,Wouters, J. (deposition date: 2021-06-30, release date: 2021-12-29, Last modification date: 2024-01-31)
Primary citationMirgaux, M.,Leherte, L.,Wouters, J.
Temporary Intermediates of L-Trp Along the Reaction Pathway of Human Indoleamine 2,3-Dioxygenase 1 and Identification of an Exo Site.
Int J Tryptophan Res, 14:11786469211052964-11786469211052964, 2021
Cited by
PubMed Abstract: Protein dynamics governs most of the fundamental processes in the human body. Particularly, the dynamics of loops located near an active site can be involved in the positioning of the substrate and the reaction mechanism. The understanding of the functioning of dynamic loops is therefore a challenge, and often requires the use of a multi-disciplinary approach mixing, for example, crystallographic experiments and molecular dynamics simulations. In the present work, the dynamic behavior of the JK-loop of the human indoleamine 2,3-dioxygenase 1 hemoprotein, a target for immunotherapy, is investigated. To overcome the lack of knowledge on this dynamism, the study reported here is based on 3 crystal structures presenting different conformations of the loop, completed with molecular dynamics trajectories and MM-GBSA analyses, in order to trace the reaction pathway of the enzyme. In addition, the crystal structures identify an exo site in the small unit of the enzyme, that is populated redundantly by the substrate or the product of the reaction. The role of this newer reported exo site still needs to be investigated.
PubMed: 34949925
DOI: 10.1177/11786469211052964
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.5 Å)
Structure validation

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