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7OKX

Structure of active transcription elongation complex Pol II-DSIF (SPT5-KOW5)-ELL2-EAF1 (composite structure)

7OKX の概要
エントリーDOI10.2210/pdb7okx/pdb
関連するPDBエントリー7OKX 7OKY 7OL0
EMDBエントリー12966 12967 12968 12969 12970 12971 12972 12973 12974
分子名称DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, RNA_pol_L_2 domain-containing protein, ... (20 entities in total)
機能のキーワードpol ii, super elongation complex, ell2, eaf1, transcription
由来する生物種Homo sapiens (Human)
詳細
タンパク質・核酸の鎖数18
化学式量合計798288.07
構造登録者
Chen, Y.,Vos, S.M.,Dienemann, C.,Ninov, M.,Urlaub, H.,Cramer, P. (登録日: 2021-05-18, 公開日: 2021-07-14, 最終更新日: 2024-07-10)
主引用文献Chen, Y.,Vos, S.M.,Dienemann, C.,Ninov, M.,Urlaub, H.,Cramer, P.
Allosteric transcription stimulation by RNA polymerase II super elongation complex.
Mol.Cell, 81:3386-3399.e10, 2021
Cited by
PubMed Abstract: The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and the subcomplex ELL2-EAF1, which stimulates RNA polymerase II (RNA Pol II) elongation. Here, we report the cryoelectron microscopy (cryo-EM) structure of ELL2-EAF1 bound to a RNA Pol II elongation complex at 2.8 Å resolution. The ELL2-EAF1 dimerization module directly binds the RNA Pol II lobe domain, explaining how SEC delivers P-TEFb to RNA Pol II. The same site on the lobe also binds the initiation factor TFIIF, consistent with SEC binding only after the transition from transcription initiation to elongation. Structure-guided functional analysis shows that the stimulation of RNA elongation requires the dimerization module and the ELL2 linker that tethers the module to the RNA Pol II protrusion. Our results show that SEC stimulates elongation allosterically and indicate that this stimulation involves stabilization of a closed conformation of the RNA Pol II active center cleft.
PubMed: 34265249
DOI: 10.1016/j.molcel.2021.06.019
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.3 Å)
構造検証レポート
Validation report summary of 7okx
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-20に公開中

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