7OKX

Structure of active transcription elongation complex Pol II-DSIF (SPT5-KOW5)-ELL2-EAF1 (composite structure)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0001054	molecular_function	RNA polymerase I activity
A	0001055	molecular_function	RNA polymerase II activity
A	0001056	molecular_function	RNA polymerase III activity
A	0001057	molecular_function	RNA polymerase IV activity
A	0001058	molecular_function	RNA polymerase V activity
A	0001059	biological_process	transcription by RNA polymerase IV
A	0001060	biological_process	transcription by RNA polymerase V
A	0001065	molecular_function	mitochondrial single subunit type RNA polymerase activity
A	0001066	molecular_function	plastid single subunit type RNA polymerase activity
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005634	cellular_component	nucleus
A	0006351	biological_process	DNA-templated transcription
A	0006360	biological_process	transcription by RNA polymerase I
A	0006366	biological_process	transcription by RNA polymerase II
A	0006383	biological_process	transcription by RNA polymerase III
A	0006390	biological_process	mitochondrial transcription
A	0016779	molecular_function	nucleotidyltransferase activity
A	0031981	cellular_component	nuclear lumen
A	0042793	biological_process	plastid transcription
A	0046872	molecular_function	metal ion binding
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0000781	cellular_component	chromosome, telomeric region
B	0001054	molecular_function	RNA polymerase I activity
B	0001055	molecular_function	RNA polymerase II activity
B	0001056	molecular_function	RNA polymerase III activity
B	0001057	molecular_function	RNA polymerase IV activity
B	0001058	molecular_function	RNA polymerase V activity
B	0001059	biological_process	transcription by RNA polymerase IV
B	0001060	biological_process	transcription by RNA polymerase V
B	0001065	molecular_function	mitochondrial single subunit type RNA polymerase activity
B	0001066	molecular_function	plastid single subunit type RNA polymerase activity
B	0001172	biological_process	RNA-templated transcription
B	0003677	molecular_function	DNA binding
B	0003682	molecular_function	chromatin binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0003968	molecular_function	RNA-dependent RNA polymerase activity
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005665	cellular_component	RNA polymerase II, core complex
B	0006351	biological_process	DNA-templated transcription
B	0006360	biological_process	transcription by RNA polymerase I
B	0006366	biological_process	transcription by RNA polymerase II
B	0006383	biological_process	transcription by RNA polymerase III
B	0006390	biological_process	mitochondrial transcription
B	0016779	molecular_function	nucleotidyltransferase activity
B	0032549	molecular_function	ribonucleoside binding
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0042793	biological_process	plastid transcription
B	0046872	molecular_function	metal ion binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005665	cellular_component	RNA polymerase II, core complex
C	0005829	cellular_component	cytosol
C	0006351	biological_process	DNA-templated transcription
C	0006366	biological_process	transcription by RNA polymerase II
C	0046983	molecular_function	protein dimerization activity
D	0000166	molecular_function	nucleotide binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005665	cellular_component	RNA polymerase II, core complex
D	0005829	cellular_component	cytosol
D	0006352	biological_process	DNA-templated transcription initiation
D	0006366	biological_process	transcription by RNA polymerase II
D	0006367	biological_process	transcription initiation at RNA polymerase II promoter
D	0016607	cellular_component	nuclear speck
D	0030880	cellular_component	RNA polymerase complex
D	0031369	molecular_function	translation initiation factor binding
D	0044237	biological_process	obsolete cellular metabolic process
E	0001054	molecular_function	RNA polymerase I activity
E	0001055	molecular_function	RNA polymerase II activity
E	0001056	molecular_function	RNA polymerase III activity
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005665	cellular_component	RNA polymerase II, core complex
E	0005666	cellular_component	RNA polymerase III complex
E	0005736	cellular_component	RNA polymerase I complex
E	0006351	biological_process	DNA-templated transcription
E	0006362	biological_process	transcription elongation by RNA polymerase I
E	0006366	biological_process	transcription by RNA polymerase II
E	0042797	biological_process	tRNA transcription by RNA polymerase III
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0001650	cellular_component	fibrillar center
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005665	cellular_component	RNA polymerase II, core complex
F	0005666	cellular_component	RNA polymerase III complex
F	0005736	cellular_component	RNA polymerase I complex
F	0006351	biological_process	DNA-templated transcription
F	0006366	biological_process	transcription by RNA polymerase II
G	0003676	molecular_function	nucleic acid binding
G	0006351	biological_process	DNA-templated transcription
G	0006352	biological_process	DNA-templated transcription initiation
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005665	cellular_component	RNA polymerase II, core complex
H	0006351	biological_process	DNA-templated transcription
I	0000428	cellular_component	DNA-directed RNA polymerase complex
I	0001193	biological_process	maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II
I	0003676	molecular_function	nucleic acid binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005665	cellular_component	RNA polymerase II, core complex
I	0005730	cellular_component	nucleolus
I	0006283	biological_process	transcription-coupled nucleotide-excision repair
I	0006351	biological_process	DNA-templated transcription
I	0006366	biological_process	transcription by RNA polymerase II
I	0006367	biological_process	transcription initiation at RNA polymerase II promoter
I	0008270	molecular_function	zinc ion binding
I	0046872	molecular_function	metal ion binding
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0006351	biological_process	DNA-templated transcription
J	0008270	molecular_function	zinc ion binding
J	0046872	molecular_function	metal ion binding
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0001055	molecular_function	RNA polymerase II activity
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005634	cellular_component	nucleus
K	0005665	cellular_component	RNA polymerase II, core complex
K	0006351	biological_process	DNA-templated transcription
K	0006366	biological_process	transcription by RNA polymerase II
K	0046983	molecular_function	protein dimerization activity
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
L	0005634	cellular_component	nucleus
L	0006351	biological_process	DNA-templated transcription
L	0008270	molecular_function	zinc ion binding
L	0046872	molecular_function	metal ion binding
M	0000987	molecular_function	cis-regulatory region sequence-specific DNA binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0006366	biological_process	transcription by RNA polymerase II
M	0006368	biological_process	transcription elongation by RNA polymerase II
M	0008023	cellular_component	transcription elongation factor complex
M	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
M	0042795	biological_process	snRNA transcription by RNA polymerase II
O	0003711	molecular_function	transcription elongation factor activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0006368	biological_process	transcription elongation by RNA polymerase II
O	0008023	cellular_component	transcription elongation factor complex
O	0015030	cellular_component	Cajal body
O	0016604	cellular_component	nuclear body
O	0016607	cellular_component	nuclear speck
O	0032783	cellular_component	super elongation complex
O	0034243	biological_process	regulation of transcription elongation by RNA polymerase II
O	0043231	cellular_component	intracellular membrane-bounded organelle
O	0045171	cellular_component	intercellular bridge
Z	0000122	biological_process	negative regulation of transcription by RNA polymerase II
Z	0003682	molecular_function	chromatin binding
Z	0003723	molecular_function	RNA binding
Z	0003729	molecular_function	mRNA binding
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005654	cellular_component	nucleoplasm
Z	0006354	biological_process	DNA-templated transcription elongation
Z	0006357	biological_process	regulation of transcription by RNA polymerase II
Z	0006368	biological_process	transcription elongation by RNA polymerase II
Z	0016239	biological_process	positive regulation of macroautophagy
Z	0019899	molecular_function	enzyme binding
Z	0032044	cellular_component	DSIF complex
Z	0032784	biological_process	regulation of DNA-templated transcription elongation
Z	0032785	biological_process	negative regulation of DNA-templated transcription, elongation
Z	0032786	biological_process	positive regulation of DNA-templated transcription, elongation
Z	0034243	biological_process	regulation of transcription elongation by RNA polymerase II
Z	0045944	biological_process	positive regulation of transcription by RNA polymerase II
Z	0046982	molecular_function	protein heterodimerization activity
Z	0140673	biological_process	transcription elongation-coupled chromatin remodeling

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DNDPSDYVEqdDI

Chain	Residue	Details
C	ASP136-ILE148

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site_id	PS00115
Number of Residues	7
Details	RNA_POL_II_REPEAT Eukaryotic RNA polymerase II heptapeptide repeat. YSPTSPA

Chain	Residue	Details
A	TYR1593-ALA1599
A	TYR1671-SER1677
A	TYR1678-SER1684
A	TYR1685-SER1691
A	TYR1692-SER1698
A	TYR1699-SER1705
A	TYR1706-SER1712
A	TYR1713-SER1719
A	TYR1720-SER1726
A	TYR1727-SER1733
A	TYR1734-SER1740
A	TYR1615-SER1621
A	TYR1741-ASN1747
A	TYR1748-ASN1754
A	TYR1755-SER1761
A	TYR1762-SER1768
A	TYR1769-ASN1775
A	TYR1776-ASN1782
A	TYR1783-SER1789
A	TYR1790-SER1796
A	TYR1797-SER1803
A	TYR1818-SER1824
A	TYR1622-SER1628
A	TYR1825-SER1831
A	TYR1832-LYS1838
A	TYR1839-SER1845
A	TYR1853-LYS1859
A	TYR1860-LYS1866
A	TYR1867-LYS1873
A	TYR1874-THR1880
A	TYR1888-THR1894
A	TYR1902-LYS1908
A	TYR1909-THR1915
A	TYR1629-ASN1635
A	TYR1916-LYS1922
A	TYR1923-THR1929
A	TYR1930-LYS1936
A	TYR1947-THR1953
A	TYR1636-SER1642
A	TYR1643-SER1649
A	TYR1650-SER1656
A	TYR1657-SER1663
A	TYR1664-SER1670

---

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YVCTAPH

Chain	Residue	Details
I	TYR112-HIS118

---

site_id	PS00446
Number of Residues	41
Details	RNA_POL_D_30KD RNA polymerases D / 30 to 40 Kd subunits signature. NSIRRvfiaevpiiAidwVqidaNsSvlhDEfIAhRLGLIP

Chain	Residue	Details
C	ASN32-PRO72

---

site_id	PS00466
Number of Residues	38
Details	ZF_TFIIS_1 Zinc finger TFIIS-type signature. CqkCghkeavffqSHSARaEDAmrlyyvCtaph.CghrW

Chain	Residue	Details
I	CYS86-TRP123

---

site_id	PS01030
Number of Residues	27
Details	RNA_POL_M_15KD RNA polymerases M / 15 Kd subunits signature. FCQECNNMLypkedkenrillyaCrnC

Chain	Residue	Details
I	PHE16-CYS42

---

site_id	PS01110
Number of Residues	14
Details	RNA_POL_H_23KD RNA polymerases H / 23 Kd subunits signature. HELVPEHvvMtkEE

Chain	Residue	Details
E	HIS142-GLU155

---

site_id	PS01111
Number of Residues	15
Details	RNA_POL_K_14KD RNA polymerases K / 14 to 18 Kd subunits signature. TkYErARvLGtRAlQ

Chain	Residue	Details
F	THR58-GLN72

---

site_id	PS01112
Number of Residues	10
Details	RNA_POL_N_8KD RNA polymerases N / 8 Kd subunits signature. IIPVrCFTCG

Chain	Residue	Details
J	ILE2-GLY11

---

site_id	PS01154
Number of Residues	32
Details	RNA_POL_L_13KD RNA polymerases L / 13 to 16 Kd subunits signature. InkEdHTLgNiIksqLlkdpqVlfagYkvpHP

Chain	Residue	Details
K	ILE35-PRO66

---

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFASrHGQKGT

Chain	Residue	Details
B	GLY1009-THR1021

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:28892040, ECO:0007744|PDB:5OIK

Chain	Residue	Details
Z	LYS579
Z	ARG619
A	ARG358
A	ARG460
A	ASP499
A	ARG1416
A	ARG1421

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:O55201

Chain	Residue	Details
Z	SER32
A	ASP495
Z	SER36
A	CYS81
A	HIS84
A	CYS111
A	CYS114
A	CYS154
A	CYS184
A	ASN493

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
Z	SER666
I	CYS20
I	CYS39
I	CYS42

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Omega-N-methylarginine; by PRMT1; alternate => ECO:0000269|PubMed:12718890

Chain	Residue	Details
Z	ARG681
Z	ARG696
I	CYS114
I	CYS119

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
Z	SER686
A	SER1868
A	SER1875
A	SER1878
A	SER1882
A	SER1896
A	SER1899
A	SER1906
A	SER1913
A	SER1917
A	SER1920
Z	SER789
A	SER1927
A	SER1931
A	SER1934
Z	SER804
A	SER1845
A	SER1849
A	SER1850
A	SER1857
A	SER1861
A	SER1864

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Symmetric dimethylarginine; by PRMT5; alternate => ECO:0000269|PubMed:12718890

Chain	Residue	Details
Z	ARG698

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:O55201

Chain	Residue	Details
Z	LYS718

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphothreonine; by CDK9 => ECO:0000269|PubMed:10757782

Chain	Residue	Details
Z	THR775
Z	THR784

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
Z	THR791
A	THR1926
A	THR1929
A	THR1933
Z	THR806
A	THR1863
A	THR1870
A	THR1877
A	THR1885
A	THR1912
A	THR1915
A	THR1919

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
Z	THR1034

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
Z	LYS143
A	LYS1866
A	LYS1873
A	LYS1887
A	LYS1922
A	LYS1936

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	TYR1860
Z	LYS1037
A	TYR1874
A	TYR1881
A	TYR1909
A	TYR1916
A	TYR1923
A	TYR1930

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P08775

Chain	Residue	Details
A	THR1894

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0000250|UniProtKB:P08775

Chain	Residue	Details
A	LYS1908

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); by NEDD4 => ECO:0000250|UniProtKB:P24928

Chain	Residue	Details
A	LYS1268

---