7N3P

Cryo-EM structure of the Cas12k-sgRNA-dsDNA complex

Summary for 7N3P

• Entry DOI: 10.2210/pdb7n3p/pdb
• EMDB information: 24143
• Descriptor: Cas12k, Single guide RNA, DNA (5'-D(*CP*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*AP*AP*CP*CP*GP*AP*GP*TP*TP*T)-3'), ... (4 entities in total)
• Functional Keywords: crispr cas, rna binding protein, protein-rna-dna complex, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: Scytonema hofmannii; More
• Total number of polymer chains: 4
• Total formula weight: 174153.07

Authors

Chang, L.,Li, Z.,Xiao, R.,Wang, S.,Han, R. (deposition date: 2021-06-01, release date: 2021-09-01, Last modification date: 2022-03-16)

• Primary citation: Xiao, R.,Wang, S.,Han, R.,Li, Z.,Gabel, C.,Mukherjee, I.A.,Chang, L.; Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.; Mol.Cell, 81:4457-4466.e5, 2021; PubMed: 34450043; DOI: 10.1016/j.molcel.2021.07.043

Experimental method

ELECTRON MICROSCOPY (3.65 Å)