7N3P
Cryo-EM structure of the Cas12k-sgRNA-dsDNA complex
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.65 |
| Number of Particle | 3021295 |
| Software | PHENIX |
| CTF correction | NONE |
