7K66
Structure of Blood Coagulation Factor VIII in Complex with an Anti-C1 Domain Pathogenic Antibody Inhibitor
Summary for 7K66
Entry DOI | 10.2210/pdb7k66/pdb |
Related | 6MF0 |
Descriptor | Coagulation factor VIII,Coagulation factor VIII,Coagulation factor VIII,Coagulation factor VIII, 2A9 heavy chain, 2A9 light chain, ... (10 entities in total) |
Functional Keywords | antibody, inhibitor, blood clotting, blood clotting-immune system complex, blood clotting/immune system |
Biological source | Sus scrofa (Pig) More |
Total number of polymer chains | 3 |
Total formula weight | 217290.01 |
Authors | Childers, K.C.,Gish, J.,Jarvis, L.,Peters, S.,Garrels, C.,Smith, I.W.,Spencer, H.T.,Spiegel, P.C. (deposition date: 2020-09-18, release date: 2020-10-14, Last modification date: 2024-11-13) |
Primary citation | Gish, J.S.,Jarvis, L.,Childers, K.C.,Peters, S.C.,Garrels, C.S.,Smith, I.W.,Spencer, H.T.,Doering, C.B.,Lollar, P.,Spiegel, P.C. Structure of blood coagulation factor VIII in complex with an anti-C1 domain pathogenic antibody inhibitor. Blood, 137:2981-2986, 2021 Cited by PubMed Abstract: Antibody inhibitor development in hemophilia A represents the most significant complication resulting from factor VIII (fVIII) replacement therapy. Recent studies have demonstrated that epitopes present in the C1 domain contribute to a pathogenic inhibitor response. In this study, we report the structure of a group A anti-C1 domain inhibitor, termed 2A9, in complex with a B domain-deleted, bioengineered fVIII construct (ET3i). The 2A9 epitope forms direct contacts to the C1 domain at 3 different surface loops consisting of Lys2065-Trp2070, Arg2150-Tyr2156, and Lys2110-Trp2112. Additional contacts are observed between 2A9 and the A3 domain, including the Phe1743-Tyr1748 loop and the N-linked glycosylation at Asn1810. Most of the C1 domain loops in the 2A9 epitope also represent a putative interface between fVIII and von Willebrand factor. Lastly, the C2 domain in the ET3i:2A9 complex adopts a large, novel conformational change, translocating outward from the structure of fVIII by 20 Å. This study reports the first structure of an anti-C1 domain antibody inhibitor and the first fVIII:inhibitor complex with a therapeutically active fVIII construct. Further structural understanding of fVIII immunogenicity may result in the development of more effective and safe fVIII replacement therapies. PubMed: 33529335DOI: 10.1182/blood.2020008940 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.92 Å) |
Structure validation
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