7JUK

Crystal structure of PTEN with a tetra-phosphorylated tail (4p-crPTEN-13sp-T2, SDTTDSDPENEG)

7JUK の概要

• エントリーDOI: 10.2210/pdb7juk/pdb
• 分子名称: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: phosphatase, phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase pten, signaling protein, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40981.40

構造登録者

Dempsey, D.,Phan, K.,Cole, P.,Gabelli, S.B. (登録日: 2020-08-19, 公開日: 2021-10-13, 最終更新日: 2023-10-18)

主引用文献

Dempsey, D.R.,Viennet, T.,Iwase, R.,Park, E.,Henriquez, S.,Chen, Z.,Jeliazkov, J.R.,Palanski, B.A.,Phan, K.L.,Coote, P.,Gray, J.J.,Eck, M.J.,Gabelli, S.B.,Arthanari, H.,Cole, P.A.
The structural basis of PTEN regulation by multi-site phosphorylation.
Nat.Struct.Mol.Biol., 28:858-868, 2021

PubMed Abstract: Phosphatase and tensin homolog (PTEN) is a phosphatidylinositol-3,4,5-triphosphate (PIP) phospholipid phosphatase that is commonly mutated or silenced in cancer. PTEN's catalytic activity, cellular membrane localization and stability are orchestrated by a cluster of C-terminal phosphorylation (phospho-C-tail) events on Ser380, Thr382, Thr383 and Ser385, but the molecular details of this multi-faceted regulation have remained uncertain. Here we use a combination of protein semisynthesis, biochemical analysis, NMR, X-ray crystallography and computational simulations on human PTEN and its sea squirt homolog, VSP, to obtain a detailed picture of how the phospho-C-tail forms a belt around the C2 and phosphatase domains of PTEN. We also visualize a previously proposed dynamic N-terminal α-helix and show that it is key for PTEN catalysis but disordered upon phospho-C-tail interaction. This structural model provides a comprehensive framework for how C-tail phosphorylation can impact PTEN's cellular functions.

PubMed: 34625746
DOI: 10.1038/s41594-021-00668-5

実験手法

X-RAY DIFFRACTION (3.15 Å)