7F1U

Crystal structure of Pseudomonas putida methionine gamma-lyase Q349S mutant with L-methionine intermediates

Summary for 7F1U

• Entry DOI: 10.2210/pdb7f1u/pdb
• Descriptor: L-methionine gamma-lyase, (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid, METHIONINE, ... (5 entities in total)
• Functional Keywords: lyase, pseudomonas putida, methionine gamma-lyase, plp enzyme
• Biological source: Pseudomonas putida (Arthrobacter siderocapsulatus); More
• Total number of polymer chains: 4
• Total formula weight: 172042.61

Authors

Okawa, A.,Handa, H.,Yasuda, E.,Murota, M.,Kudo, D.,Tamura, T.,Shiba, T.,Inagaki, K. (deposition date: 2021-06-09, release date: 2022-04-20, Last modification date: 2023-11-29)

• Primary citation: Okawa, A.,Handa, H.,Yasuda, E.,Murota, M.,Kudo, D.,Tamura, T.,Shiba, T.,Inagaki, K.; Characterization and application of l-methionine gamma-lyase Q349S mutant enzyme with an enhanced activity toward l-homocysteine.; J.Biosci.Bioeng., 133:213-221, 2022; PubMed: 34953671; DOI: 10.1016/j.jbiosc.2021.11.008

Experimental method

X-RAY DIFFRACTION (2.4 Å)