7F1U

Crystal structure of Pseudomonas putida methionine gamma-lyase Q349S mutant with L-methionine intermediates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	153.907, 152.557, 80.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.400
R-factor	0.18186
Rwork	0.180
R-free	0.21938
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o7c
RMSD bond length	0.010
RMSD bond angle	1.459
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.062	0.732
Number of reflections	74230	3415
<I/σ(I)>	19.8	2.3
Completeness [%]	98.5	93.5
Redundancy	6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M NA-K PHOSPHATE BUFFER, pH 5.6-5.8, 6-9 % PEG 6000, 0.25 M AMMONIUM SULFATE. 0.5 MM PLP, 0.5%(v/v) 2-mercaptoethanol