7F1U
Crystal structure of Pseudomonas putida methionine gamma-lyase Q349S mutant with L-methionine intermediates
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, D (A, C, D) | L-methionine gamma-lyase | polymer | 398 | 42632.6 | 3 | UniProt (P13254) | Pseudomonas putida (Arthrobacter siderocapsulatus) | MGL,Homocysteine desulfhydrase,L-methioninase |
| 2 | B (B) | L-methionine gamma-lyase | polymer | 398 | 42860.7 | 1 | UniProt (P13254) | Pseudomonas putida (Arthrobacter siderocapsulatus) | MGL,Homocysteine desulfhydrase,L-methioninase |
| 3 | E, G, H (A, C, D) | (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid | non-polymer | 378.3 | 3 | Chemie (3LM) | |||
| 4 | F (B) | METHIONINE | non-polymer | 149.2 | 1 | Chemie (MET) | |||
| 5 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 124 | Chemie (HOH) |
Sequence modifications
A, C, D: 1 - 398 (UniProt: P13254)
B: 1 - 398 (UniProt: P13254)
| PDB | External Database | Details |
|---|---|---|
| Ser 349 | Gln 349 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 349 | Gln 349 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 170758.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1284.2 | |
| All* | Total formula weight | 172042.6 |
