7DDE

Cryo-EM structure of the Ape4 and Nbr1 complex

Summary for 7DDE

• Entry DOI: 10.2210/pdb7dde/pdb
• EMDB information: 30652
• Descriptor: Aspartyl aminopeptidase 1,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein, ZINC ION (3 entities in total)
• Functional Keywords: aspartyl aminopeptidase, signaling protein, hydrolase, autophagy
• Biological source: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); More
• Total number of polymer chains: 12
• Total formula weight: 1234524.13

Authors

Zhang, J.,Ye, K. (deposition date: 2020-10-28, release date: 2021-07-14, Last modification date: 2024-05-29)

Primary citation

Wang, Y.Y.,Zhang, J.,Liu, X.M.,Li, Y.,Sui, J.,Dong, M.Q.,Ye, K.,Du, L.L.
Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1.
Embo J., 40:e107497-e107497, 2021

PubMed Abstract: In selective autophagy, cargo selectivity is determined by autophagy receptors. However, it remains scarcely understood how autophagy receptors recognize specific protein cargos. In the fission yeast Schizosaccharomyces pombe, a selective autophagy pathway termed Nbr1-mediated vacuolar targeting (NVT) employs Nbr1, an autophagy receptor conserved across eukaryotes including humans, to target cytosolic hydrolases into the vacuole. Here, we identify two new NVT cargos, the mannosidase Ams1 and the aminopeptidase Ape4, that bind competitively to the first ZZ domain of Nbr1 (Nbr1-ZZ1). High-resolution cryo-EM analyses reveal how a single ZZ domain recognizes two distinct protein cargos. Nbr1-ZZ1 not only recognizes the N-termini of cargos via a conserved acidic pocket, similar to other characterized ZZ domains, but also engages additional parts of cargos in a cargo-specific manner. Our findings unveil a single-domain bispecific mechanism of autophagy cargo recognition, elucidate its underlying structural basis, and expand the understanding of ZZ domain-mediated protein-protein interactions.

PubMed: 34169534
DOI: 10.15252/embj.2020107497

Experimental method

ELECTRON MICROSCOPY (2.26 Å)