7DDE

Cryo-EM structure of the Ape4 and Nbr1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Aspartyl aminopeptidase 1,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein	polymer	929	102615.4	12	UniProt (O36014) UniProt (Q9P792) UniProt (P0AEX9) Pfam (PF02127) Pfam (PF00569) Pfam (PF01547) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	G, V, I, X, K...	ZINC ION	non-polymer		65.4	48	Chemie (ZN)
3		water	water		18.0	1911	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O, Q, S, V, X: 7 - 473 (UniProt: O36014)

PDB	External Database	Details
Met 1	-	initiating methionine
Gln 2	-	expression tag
Leu 3	-	expression tag
His 4	-	expression tag
Gly 5	-	expression tag
Lys 6	-	expression tag
Gly 474	-	linker
Phe 475	-	linker
Lys 476	-	linker
Lys 477	-	linker
Ala 478	-	linker
Ser 479	-	linker
Ser 480	-	linker
Ser 481	-	linker
Asp 482	-	linker
Asn 483	-	linker
Lys 484	-	linker
Glu 485	-	linker
Gln 486	-	linker

A, C, E, G, I, K, M, O, Q, S, V, X: 1053 - 1129 (UniProt: Q9P792)
A, C, E, G, I, K, M, O, Q, S, V, X: 2027 - 2392 (UniProt: P0AEX9)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	1231384.5
Non-Polymers*	Number of molecules	48
	Total formula weight	3139.6
All*	Total formula weight	1234524.1

*Water molecules are not included.