7AA9

Structure of SCOC pT13/pT15 LIR motif bound to GABARAPL1

Summary for 7AA9

• Entry DOI: 10.2210/pdb7aa9/pdb
• Descriptor: Gamma-aminobutyric acid receptor-associated protein-like 1, pT13/PT15 SCOC LIR (3 entities in total)
• Functional Keywords: scoc, atg8, lir, signaling protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 12
• Total formula weight: 96425.79

Authors

Lee, R.,Mouilleron, S.,Wirth, M.,Zhang, W.,O Reilly, N.,Dhira, J.,Tooze, S. (deposition date: 2020-09-03, release date: 2021-06-16, Last modification date: 2024-10-23)

Primary citation

Wirth, M.,Mouilleron, S.,Zhang, W.,Sjottem, E.,Princely Abudu, Y.,Jain, A.,Lauritz Olsvik, H.,Bruun, J.A.,Razi, M.,Jefferies, H.B.J.,Lee, R.,Joshi, D.,O'Reilly, N.,Johansen, T.,Tooze, S.A.
Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity.
J.Mol.Biol., 433:166987-166987, 2021

PubMed Abstract: Autophagy is a highly conserved degradative pathway, essential for cellular homeostasis and implicated in diseases including cancer and neurodegeneration. Autophagy-related 8 (ATG8) proteins play a central role in autophagosome formation and selective delivery of cytoplasmic cargo to lysosomes by recruiting autophagy adaptors and receptors. The LC3-interacting region (LIR) docking site (LDS) of ATG8 proteins binds to LIR motifs present in autophagy adaptors and receptors. LIR-ATG8 interactions can be highly selective for specific mammalian ATG8 family members (LC3A-C, GABARAP, and GABARAPL1-2) and how this specificity is generated and regulated is incompletely understood. We have identified a LIR motif in the Golgi protein SCOC (short coiled-coil protein) exhibiting strong binding to GABARAP, GABARAPL1, LC3A and LC3C. The residues within and surrounding the core LIR motif of the SCOC LIR domain were phosphorylated by autophagy-related kinases (ULK1-3, TBK1) increasing specifically LC3 family binding. More distant flanking residues also contributed to ATG8 binding. Loss of these residues was compensated by phosphorylation of serine residues immediately adjacent to the core LIR motif, indicating that the interactions of the flanking LIR regions with the LDS are important and highly dynamic. Our comprehensive structural, biophysical and biochemical analyses support and provide novel mechanistic insights into how phosphorylation of LIR domain residues regulates the affinity and binding specificity of ATG8 proteins towards autophagy adaptors and receptors.

PubMed: 33845085
DOI: 10.1016/j.jmb.2021.166987

Experimental method

X-RAY DIFFRACTION (1.72 Å)