7AA9

Structure of SCOC pT13/pT15 LIR motif bound to GABARAPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97
Spacegroup name	P 32
Unit cell lengths	90.944, 90.944, 92.407
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.940 - 1.720
R-factor	0.212
Rwork	0.210
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hoi
RMSD bond length	0.002
RMSD bond angle	0.459
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.940	1.780
High resolution limit [Å]	1.720	1.720
Rmerge	0.090	0.800
Rpim	0.050	0.540
Number of reflections	90238	8924
<I/σ(I)>	7.55	1.15
Completeness [%]	99.2
Redundancy	3.9
CC(1/2)	0.990	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 1500 0.1M TRIS 8