7U0L

Crystal structure of the CCoV-HuPn-2018 RBD (domain B) in complex with canine APN

This is a non-PDB format compatible entry.

Summary for 7U0L

• Entry DOI: 10.2210/pdb7u0l/pdb
• Descriptor: Aminopeptidase N, ZINC ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (12 entities in total)
• Functional Keywords: sars-cov-2 spike, covid-19, fusion peptide, fab, viral protein, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein-immune system complex, viral protein/immune system
• Biological source: Canis lupus familiaris (dog); More
• Total number of polymer chains: 2
• Total formula weight: 132794.24

Authors

Tortorici, M.A.,Veesler, D.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2022-02-18, release date: 2022-08-24, Last modification date: 2024-11-06)

Primary citation

Tortorici, M.A.,Walls, A.C.,Joshi, A.,Park, Y.J.,Eguia, R.T.,Miranda, M.C.,Kepl, E.,Dosey, A.,Stevens-Ayers, T.,Boeckh, M.J.,Telenti, A.,Lanzavecchia, A.,King, N.P.,Corti, D.,Bloom, J.D.,Veesler, D.
Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoprotein.
Cell, 185:2279-2291.e17, 2022

PubMed Abstract: The isolation of CCoV-HuPn-2018 from a child respiratory swab indicates that more coronaviruses are spilling over to humans than previously appreciated. We determined the structures of the CCoV-HuPn-2018 spike glycoprotein trimer in two distinct conformational states and showed that its domain 0 recognizes sialosides. We identified that the CCoV-HuPn-2018 spike binds canine, feline, and porcine aminopeptidase N (APN) orthologs, which serve as entry receptors, and determined the structure of the receptor-binding B domain in complex with canine APN. The introduction of an oligosaccharide at position N739 of human APN renders cells susceptible to CCoV-HuPn-2018 spike-mediated entry, suggesting that single-nucleotide polymorphisms might account for viral detection in some individuals. Human polyclonal plasma antibodies elicited by HCoV-229E infection and a porcine coronavirus monoclonal antibody inhibit CCoV-HuPn-2018 spike-mediated entry, underscoring the cross-neutralizing activity among ɑ-coronaviruses. These data pave the way for vaccine and therapeutic development targeting this zoonotic pathogen representing the eighth human-infecting coronavirus.

PubMed: 35700730
DOI: 10.1016/j.cell.2022.05.019

Experimental method

X-RAY DIFFRACTION (3.3 Å)