7M22

Cryo-EM structure of the HCMV pentamer bound by human neuropilin 2

Summary for 7M22

• Entry DOI: 10.2210/pdb7m22/pdb
• EMDB information: 23629
• Descriptor: Envelope protein UL128, Envelope glycoprotein UL130, UL131A, ... (6 entities in total)
• Functional Keywords: hcmv pentamer, nrp2, cytomegalovirus, host receptor, viral protein, viral protein-host receptor complex, viral protein/host receptor
• Biological source: Human cytomegalovirus (HHV-5); More
• Total number of polymer chains: 4
• Total formula weight: 117364.36

Authors

Wrapp, D.,McLellan, J.S. (deposition date: 2021-03-15, release date: 2021-08-11, Last modification date: 2022-03-23)

Primary citation

Wrapp, D.,Ye, X.,Ku, Z.,Su, H.,Jones, H.G.,Wang, N.,Mishra, A.K.,Freed, D.C.,Li, F.,Tang, A.,Li, L.,Jaijyan, D.K.,Zhu, H.,Wang, D.,Fu, T.M.,Zhang, N.,An, Z.,McLellan, J.S.
Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies.
Sci Adv, 8:eabm2546-eabm2546, 2022

PubMed Abstract: Human cytomegalovirus (HCMV) encodes multiple surface glycoprotein complexes to infect a variety of cell types. The HCMV Pentamer, composed of gH, gL, UL128, UL130, and UL131A, enhances entry into epithelial, endothelial, and myeloid cells by interacting with the cell surface receptor neuropilin 2 (NRP2). Despite the critical nature of this interaction, the molecular determinants that govern NRP2 recognition remain unclear. Here, we describe the cryo-EM structure of NRP2 bound to Pentamer. The high-affinity interaction between these proteins is calcium dependent and differs from the canonical carboxyl-terminal arginine (CendR) binding that NRP2 typically uses. We also determine the structures of four neutralizing human antibodies bound to the HCMV Pentamer to define susceptible epitopes. Two of these antibodies compete with NRP2 binding, but the two most potent antibodies recognize a previously unidentified epitope that does not overlap the NRP2-binding site. Collectively, these findings provide a structural basis for HCMV tropism and antibody-mediated neutralization.

PubMed: 35275718
DOI: 10.1126/sciadv.abm2546

Experimental method

ELECTRON MICROSCOPY (3.65 Å)