6ZOO
Photosystem I reduced Plastocyanin Complex
Summary for 6ZOO
| Entry DOI | 10.2210/pdb6zoo/pdb |
| Related | 6YEZ |
| EMDB information | 11326 |
| Descriptor | Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit X psaK, ... (33 entities in total) |
| Functional Keywords | photosystem i, membrane protein, photosynthesis |
| Biological source | Pisum sativum (Garden pea) More |
| Total number of polymer chains | 17 |
| Total formula weight | 563158.92 |
| Authors | Nelson, N.,Caspy, I.,Shkolnisky, Y. (deposition date: 2020-07-07, release date: 2021-06-16, Last modification date: 2025-04-09) |
| Primary citation | Caspy, I.,Fadeeva, M.,Kuhlgert, S.,Borovikova-Sheinker, A.,Klaiman, D.,Masrati, G.,Drepper, F.,Ben-Tal, N.,Hippler, M.,Nelson, N. Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions. Biochem.J., 478:2371-2384, 2021 Cited by PubMed Abstract: Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover. PubMed: 34085703DOI: 10.1042/BCJ20210267 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.74 Å) |
Structure validation
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