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6ZG3

the structure of ECF PanT transporter in a complex with a nanobody

Summary for 6ZG3
Entry DOI10.2210/pdb6zg3/pdb
Related5JSZ 6FNP
DescriptorEnergy-coupling factor transporter ATP-binding protein EcfA1, Energy-coupling factor transporter ATP-binding protein EcfA2, Conserved hypothetical membrane protein, ... (8 entities in total)
Functional Keywordsecf transporter, vitamin transport, pantothenate, membrane protein, transport protein
Biological sourceLactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
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Total number of polymer chains10
Total formula weight271253.20
Authors
Setyawati, I.,Guskov, A.,Slotboom, D.J. (deposition date: 2020-06-18, release date: 2021-03-03, Last modification date: 2024-01-24)
Primary citationSetyawati, I.,Stanek, W.K.,Majsnerowska, M.,Swier, L.J.Y.M.,Pardon, E.,Steyaert, J.,Guskov, A.,Slotboom, D.J.
In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters.
Elife, 9:-, 2020
Cited by
PubMed Abstract: Energy-coupling factor (ECF) transporters mediate import of micronutrients in prokaryotes. They consist of an integral membrane S-component (that binds substrate) and ECF module (that powers transport by ATP hydrolysis). It has been proposed that different S-components compete for docking onto the same ECF module, but a minimal liposome-reconstituted system, required to substantiate this idea, is lacking. Here, we co-reconstituted ECF transporters for folate (ECF-FolT2) and pantothenate (ECF-PanT) into proteoliposomes, and assayed for crosstalk during active transport. The kinetics of transport showed that exchange of S-components is part of the transport mechanism. Competition experiments suggest much slower substrate association with FolT2 than with PanT. Comparison of a crystal structure of ECF-PanT with previously determined structures of ECF-FolT2 revealed larger conformational changes upon binding of folate than pantothenate, which could explain the kinetic differences. Our work shows that a minimal in vitro system with two reconstituted transporters recapitulates intricate kinetics behaviour observed in vivo.
PubMed: 33350937
DOI: 10.7554/eLife.64389
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.8 Å)
Structure validation

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