6ZG3

the structure of ECF PanT transporter in a complex with a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91587
Spacegroup name	P 1
Unit cell lengths	97.290, 110.470, 110.500
Unit cell angles	89.00, 102.27, 102.24

Refinement procedure

Resolution	48.830 - 2.800
R-factor	0.244
Rwork	0.242
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jsz
RMSD bond length	0.010
RMSD bond angle	1.286
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	104704	7625
<I/σ(I)>	4.7
Completeness [%]	96.7	95
Redundancy	1.72
CC(1/2)	0.997	0.195

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277.15	70mM citrate, 22% PEG 300