6ZG3
the structure of ECF PanT transporter in a complex with a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.290, 110.470, 110.500 |
| Unit cell angles | 89.00, 102.27, 102.24 |
Refinement procedure
| Resolution | 48.830 - 2.800 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jsz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.830 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 104704 | 7625 |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 96.7 | 95 |
| Redundancy | 1.72 | |
| CC(1/2) | 0.997 | 0.195 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277.15 | 70mM citrate, 22% PEG 300 |
